Evaluation the effect of seed priming with zinc sulfate on photosynthesis and yield components, of two varieties cowpea (Vigna unguiculata L.) under water deficit stress

Articles in Press

Document Type : Original Article

Authors

• sara raesi bidkani ¹
• Mohammad Javad Zarae ²

¹ PHD student of Ilam University

² Department of Agronomy and Crop Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran

10.22067/ijpr.2024.90074.1100

Abstract

Introduction
Cowpea is among the most important plants of the legume family, being a good source of protein, fiber, and essential nutrients. Cowpeas are also cost-effective and have high nutritional value. It has been shown that priming seeds with zinc can improve seedling vigor, growth, and overall plant performance. Priming seeds with ZnSO₄ has been found to increase plant tolerance to environmental stress such as drought and water deficiency. Overall, it has been well-documented that priming seeds with zinc can be a promising practice for improving crop productivity under environmental stresses. Considering the simplicity of this technique and the widespread use of priming seeds with nutrients, this experiment was conducted to investigate the effect of priming seeds with zinc sulfate on some physiological traits, improvement in drought tolerance, and seed yield of two cowpea varieties.
 
Materials and Methods 
The present study was conducted to investigate the effect of seed priming with zinc sulfate on the plant growth and yield performance of two different varieties of cowpea (Vigna unguiculata L.) under two different soil water content conditions. The experiment was carried out as a pot experiment in 2022 at the greenhouse of the Faculty of Agriculture, Ilam University. Treatments were replicated four times. The three main factors included the irrigation level (irrigation at 70% or 50% of the field capacity), the cowpea genotype (local and Mashhad), and the priming of seeds with zinc sulfate (hydropriming, prime level 1, prime level 2). Seeds were primed in either 0.14% or 0.28% zinc solution. ZnSO₄.7H₂O was used as the source of zinc. After surface sterilization, seeds were soaked in a solution with different concentrations of zinc sulfate (‎0.14% or 0.28%‎) for 5 hours at a ratio of 1:3 distilled seed to solution (w/v). 20 seeds were planted in each pot. Plants at the 4-5 leaf stage were subjected to well-watered and water-stressed conditions. At the 50% flowering stage, leaf samples were randomly taken from 5 plants in each pot, and various plant growth and physiological characteristics such as fresh and dry weight, plant height, leaf surface area and weight, as well as the rates of photosynthesis and transpiration, leaf relative humidity, and leakage rate were measured.
 
Results and Discussion 
The results of the analysis of variance of the present study showed that the effect of water deficiency  o some growth, physiological and biochemical traits such as leaf area and weight, photosynthesis rate, transpiration intensity, electrolyte leakage, water ratio per thousand seed weight, and harvest index was significant. Treatments affected by seed priming with zinc showed better growth performance under stress conditions than control treatments (no priming). Additionally, the effect of the variety on traits such as photosynthesis rate, and harvest index was significant.. The highest leaf weight (7.10 g)  was observed in the irrigation treatment with 70% of field capacity (FC), Mashhad variety, and prime level 2, while the lowest leaf weight (2.11 g) was recorded in the irrigation treatment with 50% of crop capacity, local variety, and hydropriming. The Mashhad cultivar exhibited a higher photosynthesis rate (4.99 μMolCO₂.m^-2.S^-1) than the local cultivar (4.65 μMolCO₂.m-2.S^-1). The application of 70% FC led to a two-fold increase in the rate of photosynthesis (6.07 μMolCO₂.m^-2.S^-1) compared to irrigation with 50% of  FC (3.56 μMolCO₂.m^-2.S^-1). The highest and lowest transpiration rates were observed under the conditions of no stress in the Mashhad cultivar (160.1 mmolH₂O.m^-2.S^-1) and under the conditions of stress in the local cultivar (0.72 mmolH₂O.m^-2.S^-1), respectively. In the condition of low irrigation, the lowest (46%) and highest (49%) percentage of electrolyte leakage were obtained in priming level 2 and hydropriming, respectively. Regarding the traits related to yield, the highest number of pods per plant (8.22) was obtained in the Mashhad variety under well-irrigation conditions (70% FC), and the lowest amount (5.56) was obtained in the local variety with 50% FC. Under well-watered conditions (70% FC), the Mashhad variety had an 18% superiority in the number of seeds per plant compared to the local variety. The highest amount of seed yield in the pot was obtained under well-watered conditions and priming with 0.28% zinc solution resulting in a 28% increase compared to 50% FC and hydroprime. The use of the Mashhad cultivar under well-watered conditions led to the production of the highest harvest index (53.85%), while applying water deficit reduced the harvest index to 39.71%.
 
Conclusion
Drought stress and water deficiency are the most significant constraints contributing to reduced bean yields in arid and semi-arid regions. The findings of the present study indicate that water deficit stress can significantly declined plant yield, but seed priming with zinc could mitigate the negative effects of water stress, particularly on the plant's physiological traits. Priming seeds with zinc resulted in improved photosynthesis and subsequently increased seed yield compared to control seeds

Keywords

• transpiration
• harvest index
• micronutrients
• electrolyte leakage

Main Subjects

• Response to biotic and abiotic stresses

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References

Abdolshahi, R., Nazari, M., Safarian, A., Sadathossini, T., Salarpour, M., & Amiri, H. (2015). Integrated selection criteria for drought tolerance in wheat (Triticum aestivum L.) breeding programs using discriminant analysis. Field Crops Research, 174, 20-29. https://doi.org/10.1016/j.fcr.2015.01.009

Ahmadi, A., Jodi, M., Tavakoli, A., & Ranjbar, M. (2009). Investigation of yield and its related morphological traits responses in wheat genotypes under drought stress and irrigation conditions. JWSS-Isfahan University of Technology, 12(46), 155-165. (In Persian with English Summary)

Alborzi, M., Safikhani, F., MASOUD, S. J., Abbaszadeh, B., & Zadeh, A. (2013). The effect of drought stress on dry mather yield and essential oils of anisum (Pimpinella anisum L.). Journal of Medicinal Plants and By-Products, 2(4), 285-291. (In Persian)

Amiri, A., Yadolahi, P., & Esmaeilzadeh, S. (2015). Effect of drought stress and chitosan and salicylic spray on morphological parameters of Carthamus tinctorius L. in Sistan. Journal of Oil Plants Production, 2(1), 43-56. (In Persian with English Summary)

Barzegari, M., Emam, Y., & Zamani, A. (2020). Yield components and grain yield responses of four wheat cultivars to growth retardant Cycocel under terminal drought stress conditions. Isfahan University of Technology-Journal of Crop Production and Processing, 10(3), 139-156. (In Persian with English Summary). http://dx.doi.org/10.47176/jcpp.10.3.20124

Borowski, E., & Michalek, S. (2011). The effect of foliar fertilization of French bean with iron salts and urea on some physiological processes in plants relative to iron uptake and translocation in leaves. Acta Scientiarum Polonorum. Hortorum Cultus, 10(2), 183-193.

Boukar, O., Togola, A., Chamarthi, S., Belko, N., Ishikawa, H., Suzuki, K., & Fatokun, C. (2019). Cowpea [Vigna unguiculata (L.) Walp.]. Advances in Plant Breeding Strategies: Legumes, 7, 201.

Cakmak, I. (2008). Enrichment of cereal grains with zinc: Agronomic or genetic biofortification? Plant and Soil, 302, 1-17.

Cheraghi Tabar, E. A. A. H. R. O. (2018). Distribution of zinc fractions in some calcareous soils of mahidashtand helilan plains of kermanshah and Ilam provinces. Journal of Water and Soil Science (Science and Technology of Agriculture and Natural Resources), 22, 12. (In Persian with English Summary). http://dorl.net/dor/20.1001.1.24763594.1397.22.4.3.8

Davoudpour, Y., Schmidt, M., Calabrese, F., Richnow, H. H., Musat, N., & Musat, N. (2020). High resolution microscopy to evaluate the efficiency of surface sterilization of Zea mays seeds. Plos One, 15(11), e0242247. https://doi.org/10.1371/journal.pone.0294203

Ebrahimian, E., & Bybordi, A. (2011). Effect of iron foliar fertilization on growth, seed and oil yield of sunflower grown under different irrigation regimes. Middle East Journal of Scientific Research, 9(5),621-625.

Ehlers, J., & Hall, A. (1997). Cowpea (Vigna unguiculata L. walp.). Field Crops Research, 53(1-3), 187-204. https://doi.org/10.1016/S0378-4290(97)00031-2

FAO, (2022). FAOSTAT Rome. [(accessed on 17 December 2021)]. Available online: https://www.fao.org/faostat/es/#data/QCL.

Farooq, M., Ullah, A., Rehman, A., Nawaz, A., Nadeem, A., Wakeel, A., Nadeem, F., & Siddique, K. H. (2018). Application of zinc improves the productivity and biofortification of fine grain aromatic rice grown in dry seeded and puddled transplanted production systems. Field Crops Research, 216, 53-62. https://doi.org/10.1016/j.fcr.2017.11.004

Fracasso, A., Telo, L., Lanfranco, L., Bonfante, P., & Amaducci, S. (2020). Physiological beneficial effect of Rhizophagus intraradices inoculation on tomato plant yield under water deficit conditions. Agronomy, 10(1), 71. https://doi.org/10.3390/agronomy10010071

Frahm, M. A., Rosas, J. C., Mayek-Pérez, N., López-Salinas, E., Acosta-Gallegos, J. A., & Kelly, J. D. (2004). Breeding beans for resistance to terminal drought in the lowland tropics. Euphytica, 136, 223-232.

Frota, K., Gonvalves, M., Lopes, L. A. R., Silva, I. C. V., & Areas, J. A. G. (2017). Nutritional quality of the protein of Vigna unguiculata L. Walp and its protein isolate. Revista Ciência Agronômica, 48(5spe), 792-798. https://doi.org/10.5935/1806-6690.20170092

Gardner, W. (1968). Availability and measurement of soil water.  Water Deficits and Plant Growth, 1(22 may), 107-135.

Ghanbarı, A. A., & Rao, I. (2013). Effects of water stress on leaves and seeds of bean (Phaseolus vulgaris L.). Turkish Journal of Field Crops, 18(1), 73-77.

Ghanbari Zarmehri, S., Moosavi, S. G., Zabihi, H. R., & Seghateslami, M. J. (2013). The effect of plant growth promoting rhizobacteria (PGPR) and zinc fertilizer on forage yield of maize under water deficit stress conditions. Journal of Applied Science, Engineering and Technology, 3(23), 3281-3290.

Harris, D., Rashid, A., Miraj, G., Arif, M., Yunas, M., & Yunas, M. (2008). ‘On-farm’seed priming with zinc in chickpea and wheat in Pakistan. Plant and Soil, 306, 3-10.

Hunter, D. A., Ferrante, A., Vernieri, P., & Reid, M. S. (2004). Role of abscisic acid in perianth senescence of daffodil (Narcissus pseudonarcissus “Dutch Master”). Physiologia Plantarum, 121(2), 313-321. https://doi.org/10.1111/j.0031-9317.2004.0311.x

Jaleel, C. A., Manivannan, P., Wahid, A., Farooq, M., Al-Juburi, H. J., Somasundaram, R., & Panneerselvam, R. (2009). Drought stress in plants: A review on morphological characteristics and pigments composition., International Journal of Agriculture and Biology, 11(1), 100-105.

Kakaei, M. (2024). The effect of planting density and supplementary irrigation on the agricultural traits of lentil (Lens culinaris Medik.) in dry conditions. Iranian Journal Pulses Research, 15(2), 129-139. (In Persian with English Summary). https://doi.org/10.22067/ijpr.2024.83555.1062

Kroner, N., Kotlarski, S., Fischer, E., Lüthi, D., Zubler, E., & Schär, C. (2017). Separating climate change signals into thermodynamic, lapse-rate and circulation effects: Theory and application to the European summer climate. Climate Dynamics, 48, 3425-3440.

Malakoti, M., & Tehrani, M. (2000). The role of micronutrients on yield and qualify increasing of crops: Tabiat Modares University Press, Tehran, Iran. 328 p.( In Persian).

Makkizadeh Tafti, M., Tavakol Afshari, R., Majnoon Hosseini, N., & Naghdi Badi, H. A. (2008). Evaluation of salinity tolerance and absorption of salt by borage (Borago officinalis L.). Iranian Journal of Medicinal and Aromatic Plants Research, 24(3), 253-262.‏ ( In Persian).

Mansouri Daneshvar, M. R., & Ebrahimi, M. (2019). An overview of climate change in Iran: Facts and statistics. Environmental Systems Research, 8(1), 1-10. https://doi.org/10.1186/s40068-019-0135-3

Marschner, H. (1995). Nutrition of higher plants. Nutrition of Higher Plants, 4(1), 375-288.

Martinez, J. P., Silva, H., Ledent, J. F., & Pinto, M. (2007). Effect of drought stress on the osmotic adjustment, cell wall elasticity and cell volume of six cultivars of common beans (Phaseolus vulgaris L.). European Journal of Agronomy, 26(1), 30-38. https://doi.org/10.1016/j.eja.2006.08.003

Mazengo, K. D., & Tryphone, G. M. (2019). Response of common bean genotypes (Phaseolus vulgaris L.) to drought for growth and yield characteristics in the southern highlands of Tanzania. International Journal of Environment, Agriculture and Biotechnology, 4(3), 643-651. http://dx.doi.org/10.22161/ijeab/4.3.8

Merwad, A. R. M., Desoky, E. S. M., & Rady, M. M. (2018). Response of water deficit-stressed Vigna unguiculata performances to silicon, proline or methionine foliar application. Scientia Horticulturae, 228, 132-144. https://doi.org/10.1016/j.scienta.2017.10.008

Mohsin, A. U., Ahmad, A. U. H., Farooq, M., & Ullah, S. (2014). Influence of zinc application through seed treatment and foliar spray on growth, productivity and grain quality of hybrid maize. JAPS: Journal of Animal & Plant Sciences, 24(5), 1494-1503.

Munjonji, L., Ayisi, K. K., Boeckx, P., & Haesaert, G. (2017). Stomatal behavior of cowpea genotypes grown under varying moisture levels. Sustainability, 10(1), 12.‏ https://doi.org/10.3390/su10010012

Munoz Perea, C. G., Terán, H., Allen, R. G., Wright, J. L., Westermann, D. T., & Singh, S. P. (2006). Selection for drought resistance in dry bean landraces and cultivars. Crop Science, 46(5), 2111-2120. https://doi.org/10.2135/cropsci2006.01.0029

Nohong, B., & Nompo, S. (2015). Effect of water stress on growth, yield, proline and soluble sugars contents of Signal grass and Napier grass species. American-Eurasian Journal of Sustainable Agriculture, 9(5), 14-22.

Posmyk, M., & Janas, K. (2007). Effects of seed hydropriming in presence of exogenous proline on chilling injury limitation in Vigna radiata L. seedlings. Acta Physiologiae Plantarum, 29, 509-517.

Prasad, A. S. (1984, October). Discovery and importance of zinc in human nutrition. Federation Proceedings, 43(13), 2829-2834.

Ramirez-Vallejo, P., & Kelly, J. D. (1998). Traits related to drought resistance in common bean. Euphytica, 99, 127-136.‏

Rezaei, H., Saadat, S., Mirkhani, R., Bagheri, Y. R., Esmaeelnejad, L., Nouri Hosseini, M., Baybordi, A., Karimi, A., Sedri, M., & Hamedi, F. (2022). The state of soil organic carbon in agricultural lands of Iran with different agroecological conditions. International Journal of Environmental Analytical Chemistry, 102(16), 4623-4639. https://doi.org/10.1080/03067319.2020.1786547

Sabzi, S., Tahmasebi, Z., & Barari, M. (2017). Study of the yield and some important plant of common bean (Phaseolus vulgaris) genotypes at different moisture levels. Environmental Stresses in Crop Sciences, 10(1), 21-30.(In Persian with English Summary). https://doi.org/10.22077/escs.1396.528

‏Saeidi Aboueshaghi, R., & Yadavi, A. (2015). Effects of irrigation levels and foliar application with iron and zinc on quantitative and qualitative traits of red bean (Phaseolous vulgaris L. Iranian Journal of Pulses Research, 6(1), 54-65. (In Persian with English Summary). https://doi.org/10.22067/ijpr.v1394i1.49008

Singh, H. P., Kaur, G., Batish, D. R., Kumar, R. K., &  Batish, D. (2012). Growth, photosynthetic activity and oxidative stress in wheat (Triticum aestivum) after exposure of lead to soil. Journal of Environmental Biology, 33(2), 265.

Sumner, M. E. (1999). Handbook of Soil Science: CRC Press, 2148 pp.

Tandon, P. (1996). Effect of zinc and iron supply on production potential of rice (Oryza sativa L.). Journal of Agronomy and Crop Science, 176(3), 213-216. https://doi.org/10.1111/j.1439-037X.1996.tb00465.x

Tatrai, Z. A., Sanoubar, R., Pluhár, Z., Mancarella, S., Orsini, F., & Gianquinto, G. (2016). Morphological and physiological plant responses to drought stress in Thymus citriodorus. International Journal of Agronomy, 2016(1), 4165750. https://doi.org/10.1155/2016/4165750

Thalooth, A., Tawfik, M., & Mohamed, H. M. (2006). A comparative study on the effect of foliar application of zinc, potassium and magnesium on growth, yield and some chemical constituents of mungbean plants grown under water stress conditions. World Journal of Agricultural Sciences, 2(1), 37-46.

Valentovic, P., Luxova, M., Kolarovic, L., Gasparikova, O., & Kolarovic, L. (2006). Effect of osmotic stress on compatible solutes content, membrane stability and water relations in two maize cultivars. Plant Soil and Environment, 52(4), 184.

Wahyudi, A., & Syukur, M. (2021). Multi-location evaluation of yield component character and proximate analysis of cowpea grown in Lampung province, Indonesia. Biodiversitas Journal of Biological Diversity, 22(10), 4246-4253. https://orcid.org/0000-0003-0442-7688

Weisany, W., Sohrabi, Y., Heidari, G., Siosemardeh, A., & Ghassemi-Golezani, K. (2011). Physiological responses of soybean ('Glycine max' L.) To zinc application under salinity stress. Australian Journal of Crop Science, 5(11), 1441-1447.

Wu, F., Bao, W., Li, F., & Wu, N. (2008). Effects of water stress and nitrogen supply on leaf gas exchange and fluorescence parameters of Sophora davidii seedlings. Photosynthetica, 46, 40-48.

Zadehbagheri, M., & Sharafzadeh, S. (2012). Effect of drought stress on yield and yield components, relative leaf water content, proline and potassium ion accumulation in different white bean (Phaseolus vulgaris L.) genotype. African Journal of Agricultural Research, 7(42), 5661-5670. https://doi.org/10.5897/AJAR10.901

Zago, M. P., & Oteiza, P. I. (2001). The antioxidant properties of zinc: interactions with iron and antioxidants. Free Radical Biology and Medicine, 31(2), 266-274. https://doi.org/10.1016/S0891-5849(01)00583-4

Zhu, J. K. (2002). Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 53(1), 247-273. https://doi.org/10.1146/annurev.arplant.53.091401.143329

Zhu, Kimbal, B., Cheng, L., Kobayashi, K., & Bindi, M. (2002). Responses of agricultural crops to free-air CO2enrichment. Yingyong Shengtai Xuebao, 13, 1323-1338. https://doi.org/10.1016/S0065-2113(02)77017-X