Distribution study of resistant genes to Bean Common Mosaic Virus in bean genotypes (Phaseolus vulgaris L.) using SCAR molecular markers

Document Type : مقالات پژوهشی

Authors

• Mohammad Mojtaba Kamelmanesh ¹
• Anita Namayandeh ¹
• Mohammadreza Bihamta ²

¹ Islamic Azad University, Shiraz-Iran

² Tehran University

10.22067/ijpr.v1393i1.46256

Abstract

In order to determine resistance genetically potential of some bean genotypes to Bean Common Mosaic Virus, two separated experiments in randomized complete block design with 25 genotypes and three replications was conducted in IAU Shiraz Branch. The first experiment and the second one were performed in normal and BCMV infected conditions, respectively. Plants were inoculated in cotyledon leaf and first triple leaflet stage in the method of mechanical using carborundum powder. After 21 days, the first inoculation, sampling for PTA-ELISA test was done. According to the results, the genotypes that have I gene and at least one of the bc-3 and bc-12 genes showed less yield decline in BCMV infected conditions than other genotypes (p-value=0.001). The least yield variation was relevant to WA8528-9 genotype (5.67%) which containing of I and bc-3 genes. Among the genotypes, only 11805 had all of three (I, bc-3 and bc-12 ) genes. Yield variation of this genotype was 9.05%. Evidences have shown that 60% of genotypes had I, 44% had bc-3 and 8% had bc-12 genes, respectively. One of the other important results of this research was negative reaction of two genotypes (Local Khomein and Capsoli) to ELISA test. These genotypes were devoid of each three resistant genes, whereas yield variations of them were low in BCMV infected conditions. Existence of new combinations of genes are possible in Local Khomein and Capsoli genotypes, so these genotypes should be considered specially.

Keywords

• BCMV
• Bean
• SCAR marker
• Yield variation

---

References

1. Ali, M. 1950. Genetic of resistance to the common bean mosaic virus (bean virus 1) in the bean (Phaseolus vulgaris L.). Phytopathology 40: 69-79.

2. Ariyarathne, H.M., Coyne, D.P., Jung, G., Skroch, P.W., Vidaver, A.K., Steadman, J.R., Miklas, P.N., and Bassett, M.J. 1999. Molecular mapping of disease resistance genes for halo blight, common bacterial blight, and bean common mosaic virus in a segregating population of common bean. Journal of the American Society for Horticultural Science 124(6): 654-662.

3. Clark, M.F., and Adams, A.N. 1977. Characteristics of the micro plate method of enzyme-linked immune sorbent assay for detection of plant viruses. Journal of Genetic Virology 34: 475-483.

4. Collmer, C.W., Marston, M.F., Taylor, J.C., and Jahn, M. 2000. The I gene of bean: A dosage-dependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus bean common mosaic virus. Molecular Plant-Microbiology Interaction 13: 1266-1270.

5. Day, K.L. 1983. Resistance to bean common mosaic virus in Phaseolus bean. Annual Report pp: 19- 21.

6. Drijfhout, E. 1978. Genetic interact Phaseolus vulgaris and bean common mosaic virus with implications for strain identification and breeding for resistance. PhD. Dissertation. Wageningen Agriculture Research Report. 872.

7. Drijfhout, E. 1991. Bean Common Mosaic. In: R. Hall (Ed.). Compendium of Bean Diseases. APS Press, The American Phytopathological Society, Minnesota: 37-39.

8. Drijfhout, E., Silbernagel, M.J., and Burke, D.W. 1978. Differentiation of strains of bean common mosaic virus. Netherland Journal Plant Pathology 84: 13-26.

9. Eduardo, C.V., Gustavo, A.M., Valerie, J., Tommy, R.P., Ney, S.S., and Sally, A.M. 2006. Genetic and molecular characterization of the I locus of Phaseolus vulgaris. Genetics 172: 1229-1242.

10. Gowel, N.J., and Jarret, R.L. 1991. A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Molecular Biology Report 9: 262-266.

11. Haley, S.D., Afanador, L., and Kelly, J.D. 1994. Identification and application of a random amplified polymorphic DNA marker for the I gene (potyvirus resistance) in common bean. Phytopathology 84: 157-160.

12. Hassani, M., and Shahraeen, N. 2001. Determine of resistance bean genetically sources to CMV, BYMV and BCMV. Final Report, Central Province Agriculture Research Center.

13. Johnson, W.C., Guzman, P., Mandala, D., Mkandawire, A.B.C., Temple, S., Gilbertson, R.L., and Gepts, P. 1997. Molecular tagging of the bc-3 gene for introgression into Andean common bean. Crop Science 37: 248-254.

14. Kamelmanesh, M.M., Dorri, H.R., Ghasemi, S., Bihamta, M.R., and Darvish, F. 2008. Gene action for resistance to Bean common mosaic virus (BCMV) in common bean (Phaseolus vulgaris L.). Iranian Journal of Field Crops Research 6(2): 363-370.

15. Kelly, J.D. 1997. A review of varietal response to bean common mosaic potyvirus in Phaseolus vulgaris. Plant & Seed 10: 1-6.

16. Kelly, J.D., Afanador, L., and Haley, S.D. 1995. Pyramiding genes for resistance to bean common mosaic virus. Euphytica 82: 207-212.

17. Makeshimana, G., Pandea, A., Rodriguez, S.C., and Ferreira, J.J. 2005. Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean. Euphytica 144(3): 291- 299.

18. Melloto, M., Afanador, L., and Kelly, J.D. 1996. Development of a SCAR marker linked to the I gene in common bean. Genome 39: 1216-1219.

19. Miklas, P.N., Hang, A.N., Kelly, J.D., Strausbaugh, C.A., and Forster, R.L. 2002. Registration of three kidney bean germplasm lines resistance to bean common mosaic and necrosis potyvirus: USLK-2 light red kidney, USDK-4 dark red kidney, and USWK-6 white kidney. Crop Science 42: 674-675.

20. Miklas, P.N., Larsen, R.C., Riley, R., and Kelly, J.D. 2000. Potential marker-assisted selection for bc-1² resistance to bean common mosaic potyvirus in common bean. Euphytica 116: 211-219.

21. Prasad, S.M., Barnwal, M.K., Sharma, R.B., and Prasad, N. 2007. Influence of different dates of sowing on incidence of virus diseases of French bean. 18(1): 44- 46.

22. Shree, P.S. 2001. Broadening the genetic base of common bean cultivars: A review. Crop Science 41: 1659-1675.

23. Silbernagel, M.J., Mink, G.L., Zhao, R.L., and Zhang, G.Y. 2001. Phenotypic recombination between bean common mosaic virus and bean common mosaic necrosis potyvirus in vivo. Archives of Virology 146: 1007-1020.

24. Singh, S.P., Teran, H., Lema, M., Webster, D.M., Strausbaugh, C.A., Miklas, P.N., Schwartz, H.F., and Brick, M.A. 2007. Seventy-five years of breeding dry bean of the Western USA. Crop Science 47(3): 981-989.

25. Strausbaugh, C.A., Miklas, P.N., Singh, S.P., Myers, J.R., and Forster, R.L. 2003. Genetic characterization of differential reaction among host group 3 common Bean cultivars to NL-3 K strain of Bean common mosaic necrosis virus. Phytopathology 93: 683-690.

26. Strausbaugh, C.A., Myers, J.R., Forster, R.L., and Mc Clean, P.E. 1999. Bc-1 and Bc-u Two loci controlling bean common mosaic virus resistance in common bean are linked. Journal of American Society Horticulture Science 124: 644-648.