In order to study the biological fixation efficiency of different strains of symbiosis bacteria in three varieties of common bean (Phaseolus vulgaris L.) a factorial experiment based on randomized complete block design was conducted in Zanjan province during 2006 and 2007. In this investigation three strains of Rizobium including: Rb117 (extracted from the Zanjan soils), Rb123 (extracted from the Hamadan soils), Rb136 (extracted from the Chahar Mahal Bakhtiyari soils) and a biological inoculants (Rhizobean), with two nitrogen fertilizer treatments (application of 100 kg/ha N and no N application as control), and three bean cultivars (erect type) (COS16, Akhtar and Derakhshan) arranged and data analysis preformed using SAS9.1 and means comparison were done with Duncan’s Multiple Range Test. Results showed that Rb117 strain caused 59% increasing in seed yield compared with non inoculants treatments. The results showed that significant difference in seed and protein yield, seed protein percentage, number and weight of nodules (50 day after emergence) were observed among seed inoculated and non-inoculated. The highest protein content (about 24%) and protein yield (864 kg/ha) were achieved from 100 kg nitrogen, inoculated by Rb117 strain and lowest protein yield was produced from control. Among of all inoculants Rhizobium leguminosarum;bv.phaseoli, Rb117 strain was more effective than other strains, on common bean yield. Among three cultivars, COS16 line was successful in compared with other cultivars. Among Rizobium strains, Rb117 and among bean cultivars, COS16 produced the highest seed yield. Common bean production using strains of Rizobium caused 43% yield increasing and Rb117 strain compatibility was more than other strains and had the most influence on yield.