Interaction effects of salinity and salicylic acid on germination, growth and some morpho-physiological characteristics of chickpea genotypes (Cicer arietinum L.)

Document Type : Original Articles

Authors

• Ali Ganjeali ¹
• Parvaneh Abrishamchi ²
• Maryam Shoryabi ²

¹ Ferdowsi University of Mashhad, Iran

² FUM

10.22067/ijpr.v7i2.44059

Abstract

Introduction
Salinity has various effects on plant growth by affecting physiological processes. The decline in plant productivity under saline condition, frequently linked with the reduction of photosynthetic capability. It has been reported that efficiency of PSII photochemistry (Fv/Fm) of numerous plant species have been decreased due to salt stress studies have shown that salicylic acid (SA( improves resistance of plants to environmental stresses (heat, cold, drought and salt stress). In this relation, convincing data have showed that the SA-induced enhances resistance of plants to the salinity. Therefore, the present study was conducted to estimate salicylic acid effects on germination, growth and some physiological characteristics of two chickpea genotypes (Cicer arietinum L.) in salt stress condition.

Materials and Methods
In this research we studied the effects of different concentrations of SA (0.5 and 1mM), on chickpea genotypes (MCC414, MCC789) during growth stages (germination, seedling and flowering) under salinity stress. Five levels of salinity including 0, 3, 5, 10 and 12 dsm-1 in germination stage and three levels of salinity (0, 4 and 8 dsm-1) at seedling and flowering stages) were employed separately in a factorial experiment based on Randomized Completely Block Design with three replications.

Results and Discussion
Results of means comparison showed that germination rate, significantly decreased under salt stress in both genotypes (P≤0.05). Under salt stress, increasing the osmotic pressure and the reduction seeds water absorption and the toxic effects of sodium and chlorine ions, can affect seed germination. Exogenouse application of SA concentrations (0.5 mM), under salt stress (3 and 12 dsm-1) increased rate and percentage of germination in MCC789 genotype. For MCC414 genotype, the combination of the treatment with salicylic acid (0.5 and 1 mM) and salinity (10 dsm-1) increased significantly germination rate. In same genotype, radicle and coleoptile length increased significantly after treating with SA (1mM) in salt stress (12 dsm-1). Exogenous application of SA concentration (0.5 mM), under salt stress (3 dsm-1) increased coleoptile length in MCC789 genotype (P≤0.05). Seed priming with SA leads to an activation of germination and seedling growth, while the enhancement of the division of root apical cells are important contribution to the growth stimulating effect of SA. In seedling stage, salinity decreased stem and root dry weight. SA and on some salinity levels, significantly increased stem dry weight in MCC789 and total root length in MCC414 genotype (P≤0.05). The decline in plant productivity under saline condition is frequently linked with the reduction in leaf area and stomatal conductance. Positive effects of SA on growth characteristics, in wheat, barley and maize have been reported. In both genotype, chlorophyll index, transpiration rate, membrane stability index and efficiency of PSII photochemistry significantly decreased and stomatal resistance increased under salt stress in flowering stage. Serious reduction in stomatal conductance and transpiration rate are adaptive mechanisms under salt stress. Halophytes such as Beta vulgaris and Spartina townsendi have salinity tolerance and show a combination of low photosynthesis, minimal transpiration, high stomatal resistance and low internal CO2 concentrations. Studies showed chlorophyll contents decreased under saline condition. Biochemical analysis of leaves of different maize cultivars for proline and chlorophyll contents indicated that proline accumulation increased and chlorophyll contents decreased under saline condition. SA application increased chlorophyll index and membrane stability index respectively in MCC789 and MCC414 genotypes. In both genotypes, stomatal resistance, significantly decreased and efficiency of PSII photochemistry increased with SA treatment under salt stress (12 dsm-1) (P≤0.05). Studies have shown that salicylic acid protects membranes by increasing polyamines such as putrescine, spermine, spermidine, as well as membrane sustainable complexes. In wheat, SA application improves the Fv/Fm. Szepesi et al, (2005) reported SA (10-6 and 10-7 M) could increase Fv/Fm in tomato plants under drought stress.

Conclusion
The results indicated that the modulator effects on salicylic acid on germination and other physiological processes of chickpea genotypes under salt stress.

Key words: Chickpea (Cicer arietinum L.), Efficiency of PSII photochemistry, Growth parameters, Membrane stability index, Salicylic acid, Salt stress

Keywords

• Chickpea (Cicer arietinum L.)
• Efficiency of PSII photochemistry
• growth parameters
• Membrane stability index
• salicylic acid
• Salt stress

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References

1. Ahmed, S., Nawata, E., Hosokawa, M., Domae, Y., and Sakuratani, T. 2002. Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Science 163: 117-123.

2. Allakhverdiev, S.I., Sakamoto, A., Nishiyama, Y., Inaba, M., and Murata, N. 2000. Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp. Plant Physiology 123: 1047-1056.

3. Allen, R.D. 1995. Dissection of oxidative stress tolerance using transgenic plants. Plant Physiology 57: 1049-1054.

4. Asish Kumar Paridaa, A., and Anath Bandhu Das, A. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety 60: 324-349

5. Chookhampaeng, S. 2011. The Effect of salt stress on growth, chlorophyll content proline content and antioxidative enzymes of pepper (Capsicum annuum L.) seedling. European Journal of Scientific Research 49: 103-109.

6. De, F., and Kar, R.K. 1994. Seed germination and seedling growth of mung bean (Vigna radiate) under water stress induced by PEG-6000. Seed Science and Technology 23: 301-304.

7. Delany, T.P., Uknes, S., Vernooij, B., Friedrich, L., Weymann, K., Negrotto, D., Gaffney, T., Gut-Rella, M., Kessmann, H., Ward, E., and Ryals, J. 1994 . A central role of salicylic acid in plant disease resistance. Science 266: 1247-1250.

8. Demir, I., and Mavi, K. 2008. Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages. Brazilian Archives of Biology and Technology 51: 897-902.

9. Du, Y.C., Nose, A., Wasano, K., and Ushida, Y. 1981. Responses to water stress of enzyme activities and metabolite levels in relation to sucrose and starch synthesis, the Calvin cycle and the C4 pathway in sugarcane (Saccharum sp). Australian Journal of Plant Physiology 25: 253-260.

10. El-Tayeb, M.A. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation 45: 215-224.

11. Erik, T.N., and David, M.O. 1996 . The Physiology of Plants Under Stress, Abiotic Factors . Copyright by John Wiley, sons. Inc

12. FAO 2005. Salt affected soils from sea water intrusion: strategies for rehabilitation and management. Report of the Regional Workshop. Bangkok, Thailand, 62 pp.

13. Farooq, M., Aziz, T., Basra, S.M.A., Cheema, M.A., and Rahman, H. 2008. Chilling tolerance in hybrid maize induced by priming whit salicylic acid. Agronomy Crop Science 194: 161-168.

14. Gutie´rrez-Coronado, M.A., Trejo-Lopez, C., and Larque´-Saavedra. 1998. Effects of salicylic acid on growth of roots and shoots in soybean. Plant Physiology and Biochemistry 36: 653-665.

15. Hanan, E.D. 2007. Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Biological Research 1: 40-48.

16. Hayat, S., Ali, B., and Ahmad, A. 2007. Salicylic acid: biosynthesis, metabolism and physiological role in plants. Department of Botany, Aligarh Muslim University, Aligarh, INDIA 46: 1-14.

17. Hernandez, J.A., Olmos, E., Corpas, F.J., Sevilla, F., and Del Rio, L. 1995. Salt-induced oxidative stress in chloroplasts of pea plants. Plant Science 105: 151-167.

18. Hussain, M., Farooq, M., Jabran, K., and Wahid, A. 2009. Foliar application of Glycine-betaine and Salicylic acid improves growth, yield and water productivity of hybrid Sunflower planted by different sowing methods. Australian Journal of Basic & Applied Science 196(2): 136-145.

19. Kalaji, M.H., and Guo, P. 2008. Chlorophyll fluorescence: a useful tool in barley plant breedingprograms. In: A. Sanchez and S.J. Gutierrez (Eds.). Photochemistry Research Progress. Nova Publishers, NY, USA: 439-463.

20. Welfare, K., Yeo, A.R., and Flowers, T.J. 2002. Effects of salinity and ozone, individually and in combination, on the growth and ion contents of two chickpea (Cicer arietinum L.) varieties. Environmental Pollution 120: 397-40.

21. Khan, N.A. 2003. NaCl inhibited chlorophyll synthesis and associated changes in ethylene evolution and antioxidative enzyme activities in wheat. Plant Biology 47: 437-440.

22. Khodary, S.E.A. 2004. Effect of Salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed msaize plants. International Journal of Agriculture and Biology 6: 5-8.

23. Kim, S.T., Kim, S.G., Hwang, D.H., Kang, S.Y., Koo, S.C., Cho, M.J., and Kang, K.Y. 2004. Expression of a salt-induced protein (SALT) in suspension-cultured cells and leaves of rice following exposure to fungal elicitor and phytohormones. Plant Cell Report 23: 256-262.

24. Koyro, H.W. 2003. Study of potential cash crop halophytes in a quick check system task. Vegetable Science 38: 5-17.

25. Levitt, J. 1980. Responses of Plants to Environmental Stresses (Physiological Ecology): Chilling, Freezing, and High Temperature Stresses .Academic Press, New York.

26. Liu, D., Jiang, W., and Gao, X. 2003. Effect of cadmium on root growth, cell division and nucleolic in root tipe cells of garlic. Biological Plant 47(1): 79-83.

27. Molazem, D., Qurbanov, E.M., and Dunyamaliyev, S.A. 2010. Role of proline, Na and chlorophyll content in salt tolerance of corn (Zea mays L.). American-Eurasian Journal of Agricultural & Environmental Science 9(3): 319-324.

28. Najafian, S.M., and Khoshkliui, V. 2009. Effect of salicylic acid and salinity in thyme (Thymus vulgaris L.): Investigation on changes in gas exchange, water relations and membrane stabilization and biomass accwnulation. Australian Journal of Basic and Applied Sciences 3(23): 2620-2626.

29. Nemeth, M., Janda, T., Horvath, E., Paldi, E., and Szalai, G. 2002. Exogenous salicylic acid increase polyamine content but may decrease drought tolerance in maize. Plant Science 162 :569-574.

30. Pancheva, T.V., Popova, L.P., and Uzunova, A.M. 1996. Effect of salicylic acid on growth and photosynthesis in barley plants. Journal of Plant Physiology 149: 57-63.

31. Parida, A.K., and Das, A.B. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety 60: 324-349.

32. Popova, L.P., Maslenkova, L.T., Yordanova, R.Y., Ivanova, A.P., Krantev, A.P., Szalai, G., and Janda, T. 2009. Exogenous treatment with salicylic acid attenuates cadmium toxicity in pea seedlings. Plant Physiology and Biochemistry 47: 224-231.

33. Popova, L., Ananieva, V., Hristova, V., Christov, K., Geovgieva, K., Alexieva, V., and Stoinova, Z. 2003. Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of Plant Physiology (Special issue): 133-152.

34. Rehman, S., Harris, P.J.C., Bourne, W.F., and Wikin, J. 1996. The effect of sodium chloride on germination and the potassium and calcium contents of Acacia seeds. Seed Science and Technology 25: 45-57.

35. Rock, C.D. 2000. Pathways to abscisic acid-regulated gene expression. New Phytologist 148: 357-396.

36. Sairam, R.K., Veerabhadra Rao, K., and Srivastava, G.C. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science 163: 1037-1046.

37. Sairam, R., and Saxena, K. 2000. Oxidative stress and antioxidant in wheat genotypes: possible mechanism of water stress tolerance. Journal of Agronomy and Crop Science 184(1): 55-61.

38. Senaratna, T., Touchell, D., Bunn, E., and Dixon, K. 2000. Acetyl salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation 30: 157-161.

39. Sevengor, S., Yasar, F., Kusvuran, S., and Ellialtioglu, S. 2011. The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. African Journal of Agricultural Research 21: 4920-4924.

40. Shabala, S., Babourina, O., and Newman, I. 2000. Ionspecific mechanisms of osmoregulation in bean mesophyll cells. Journal of Experimental Botany 51: 1243-1253.

41. Shakirova, F.M., Sakhabutdinova, A.R., Bezrukova, M.V., Fatkhutdinova, R.A., and Fatkhutdinova, D.R. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science 164: 317-322.

42. Singh, A.K., and Dubey, R.S. 1995. Changes in chlorophyll a and b contents and activities of photosystems I and II in rice seedlings induced by NaCl. Photosynthetica 31: 489.

43. Szepesi, Á., Csiszar, J., Bajkan, Sz., Gemes, K., Horvath F., Erdei, L., Deer, A., Simon, L.M., and Tari, I. 2005. Role of salicylic aicd pre-treatment on the acclimation of tomato plants to salt- and osmotic stress. Acta Biologica Szegediensis 49: 123-125.

44. Ungar, I.A. 1995. Seed germination and seed-bank ecology of halophytes. In: J. Kigel and G. Galili (Eds). Seed Development and Germination, Marcel and Dekker Inc., 1995.

45. Wahid, A., Perveen, M., Gelani, S., and Basra, S.M.A. 2007. Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. Journal of Plant Physiology 164: 283-294.

46. Waseem, M., Athar, H.U.R., and Ashraf, M. 2006. Effect of salicylic acid applied through rooting medium on drought tolerance. Pakistan Journal of Botany 4(38): 1127-1136.

47. Zekri, M., and Parsons, L.R. 1990. Response of split-root sour orange seedlings to NaCl and polyethylene glycol stresses. Journal of Experimental Botany 41(222): 35-40.