The effects of zice fertilizer on some physiological characteristics of chickpea (Cicer arietinum L.) under water stress

Document Type : Original Articles

Authors

• nader Dadkhah ¹
• ali ebadie ²
• ghasem parmoon ³
• Ebdolghaiom Ghlipoori ³
• soodabe Jahanbakhsh ³

¹ University of Mohaghegh Ardabili, Ardabil

² Faculty of Agriculture, University of Mohaghegh

³ University of Mohaghegh Ardabili

10.22067/ijpr.v1394i2.35619

Abstract

Introduction
Dehydration is one of the limiting factors of crop production. It results from increasing the amount of water losses from leaf or inadequate water absorption rate or combination of both. Zinc used in many enzymes of the plant tissues and have catalytic role in activation of some enzymes. Zinc is involved in the synthesis and degradation of proteins and is an essential micronutrient for normal growth and reproduction of crops. It plays an important role in the synthesis of protein and carbohydrates, metabolic functions of the cell membrane to protect cells against free radicals of oxygen and other processes related to the plants adaptation to stress. Osmotic adjustment is one of the most important mechanisms of drought tolerance in plants. By this mechanism the osmotic potential of plant cells reduces by solute accumulation. Torgur potential maintains high which is essential in cell development and growth.

Materials and Methods
Effect of water deficit stress and foliar application of zinc on physiological characteristics of chickpea was studied at a factorial experiment using randomized complete block design in field crop research station of University of Mohaghegh Ardabili in 2012. Irrigation schedules consisted of three levels (1: irrigation at planting, 2 : planting + before flowering and 3 : planting + before flowering + pod set) and zinc was applied on three concentration (0, 3 and 6 kg ha-1 as zinc sulfate) in two stages of 15 and 25 days after planting. In this study Proline, Lysine, Methionine, soluble sugars, and protein content were measured in three stages 33, 55 and 75 day after stress induction and osmotic potential in 75 days after stress. Osmotic potential was measured based on the electrical conductivity, the extraction of soluble sugars by using phenol sulfuric method, total protein from the leaves by the Bradford method and Lysine and methionine assayed using Ferrel method. Data were analyzed using the SAS and means compared by the LSD at the 5% level.

Results and Discussions

The results showed that dehydration increased proline, lysine, methionine, protein and soluble sugar content but reduced the osmotic potential. The maximum amount of proline accumulation resulted in two and three stage samplings once irrigation and its minimum rate obtained in the three irrigation schedule. It seems that by reducing soil moisture availability or increasing irrigation intervals, the water potential of the cells has been reached to the lower threshold which enhanced the protease enzyme activity resulted in proline accumulation in order to enhance water absorption. The maximum amount of lysine during 55 and 75 days after the stress obtained from one irrigation. Unlike the lysine, methionine content reduced during stress period, so that it's maximum value was obtained over 55 and 75 days after the tension of 3 times irrigation. Dehydration reduced the amount of proteins so that the maximum amount of protein production observed with three irrigations. Spraying six kg ha-1 zinc sulphate also enhanced the amount of proline, soluble sugar and osmotic potential but had no effect on the amount of lysine and methionine. Correlation showed that by increasing the time from withholding irrigation by 33 days proline increased, but osmotic potential affected only after 75 days. The regression results also showed that in the early stages of sampling, proline content had the significant effect on the biomass prediction but the elapsed time increased the effect of lysine, methionine and protein on predicting the osmotic potential.


Conclusions
In general it can be said increasing the secondary metabolites production can reduce the stress and proline is the most sensitive to the low level of stress, but under higher levels of stress many metabolites such as lysine and methionine were also used for osmoregulation. The regression results also showed that in the early stages of sampling, proline levels had the largest effect on the biomass prediction, but with increasing time the lysine, methionine and protein levels also predicted the osmotic potential.

Keywords

• Correlation
• Lysine
• Proline
• Soluble sugar

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References

1. Abbas-Zade, B., Sharifi. A., Abadi, A., Lebaschi, M.H., Naderi, M., and Maghdami, F. 2007. Effect of drought stress on proline, soluble sugars, chlorophyll and relative water content of Melissa officinalis L. Journal Reserche Aroma Plants Iran 23(4): 504-513.

2. Abu-Kpawoh, J.C., Xi, Y.F., Zhang, Y.Z., and Jin, Y.F. 2002. Polyamine accumulation following Hot-water dips influence chilling injury and decay in friar plum fruit. Food Chemistry and Toxicology 67(7): 2649-2653.

3. Aziz zade Firozy, F., Bahman Yar, A., Momeny, V., and Ghasem por A. 2004. Effect of potassium fertilizers on the agronomic characteristics and quantities of zinc, iron and phosphorus in two wheat cultivars on calcareous soil with low. Proceedings of the 10th Congress of Iran, Karaj. (In Persian).

4. Babania, M., Haidary, M., and Ghanbari, A., 2010. Effects of water stress and foliar application of micronutrients on physiological characteristics and nutrient uptake of sunflower. (Helianthus annus L.). Iranian Journal of Crop Sciences 12(4): 377-391. (In Persian).

5. Banks, L.W. 2004. Effect of timing of foliar zinc fertilizer on yield component of soybeans. Australian Journal of Experimental Agriculture and Animal Husbandry 22(116): 226-231.

6. Bates, I.S., Waldern. R.P., and Teare, I.D. 1973. Rapid determination of free prolin for water stress studies. Plant and Soil 39: 205-207.

7. Bokhari, U.G., and Trent, J.D. 1985. Proline concentrations in water stressed grasses. Journal of Range Management 38: 37-38.

8. Bradford, M.M. 1976. A rapid and sensitive method for quantitation of microgram quantitie of protein utilysineg the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.

9. Brown, P.H., Cakmak, I., and Zhang, Q. 1993. Form and function of zinc in plants, In: Robson, A.D. (Ed.). Pp: 93-106.

10. Dehqanzadeh, H., Khajehpour, M.R., Heidari Sharif Abad, H., and Soleimani, A.S. 2008. Effect of limited irrigation on the accumulation of proline, free soluble sugars and potassium in bread wheat cultivars. 10th Iran. Cong. Agron. and Plant Breed Science. 430p.

11. Ferrel, R.E., Fellers, D.A., and Shepherd, A.D. 1969. Determination of free Lysine and Methionine in Amino acid-Forttified wheat. 46: 614- 620.

12. Ghorbanli, M., and Niakan, M. 2005. Study the effect of drought stress on soluble sugars, protein, proline, phenol compounds and reductase enzyme activity in soybean plants cv. Gorgan 3. Tarbiat Moallem University Science Magazin. 5(1, 2): 537-550.

13. Hajebi, A., and Hidary sharifabady, H. 2005. Effect of drought on growth and nodulation of three species of clover. Journal of Agronomy and Horticulture Research and Development 66: 13-21. (In Persian).

14. Ho, S., Chao, Y., Tong, W., and Yu, S. 2001. Sugar coordinately and differentially regulates growth and stress-related gene expression via a complex signal transduction network and multiple control mechanisms. Plant Physiology 46: 281-285.

15. Irigoyen, J. J., Emerich D.W., and Sanchez-Diaz, M. 1992. Alfalfa leaf senescense induced by drought stress: photosnthesis, hydrogen peroxide metabolism, lipid peroxidation and athylene evoluation. Physiologia Plantarum 84: 67-72.

16. Jamson, M., Galshy, S., Pahlevani, M., and Zinaly, A., 2009. Effect of foliar zinc (Zn) on the yield and grain quality characteristics of soybean cultivars in summer cultivation. Journal of Plant Production 16(1): 17-28. (In Persian).

17. Janardhan, K.V., and Krishnamorthy, V. 1975. Arapid metod for determination of osmotic potencial of plant cell. Curren Science 44(11): 390-391.

18. Khan, A.S., Singh, Z., Abbasi, N.A., and Swinny, E.E. 2008. Pre or post-harvest application of putrescine and low temperature storage affect fruit ripening and quality of Agelino plum. Journal of the Science of food and Agriculture 88(11): 1687-1695.

19. Liu, J. H., Kitashiba, H., Wang, J., Ban, Y., and Moriguchi, T. 2007. Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnology 24: 117-126.

20. Loos, S.P., and Siddique, K.H.M. 1994. Morphological and physiological traits Associated with wheat increases in Mediterranean environment. Adv. in Agronomy 52: 229-276.

21. Mansour, M.M.F., Salama, K.H., Ali, F.Z.M., and Abou Hadid, A.F. 2005. Cell and plant responses to NaCl in Zea Mays L. cultivars differing in salt tolerance. Gentice Applec Plant Physiology 31(1-2): 29-41.

22. Marschner, H. 1995. Mineral Nutrition of Higher Plants, 2. Academic Press, London, U.K., Pp. 889.

23. Mazhar, U., Haq, M.U., and Mallarino, A.P. 2005. Response of soybean grain oil and protein concentrations to foliar and soil fertilization. Agronomy Journal 97: 910-918

24. Mohsenzade, S., Malboobi, M.A., Razavi, K., and Farrahi Aschtiani, S. 2006. Physiological and molecular responses of Aeluropus lagopoides (poaceas) to water deficit. Environmental and Experimental Botany 56: 374-322.

25. Moran, J.F., Becana, M., Ormaetxe, I.I., Frechilla, S.L., Klucasc, R.V., and Tejo, D.A. 1994. Drought induces oxidative stress in pea plants. Plant 194: 346-352.

26. Murray, D. R. 1989. Biology of fool irradiation. Research Studies Press, UK. Esmith, D. S. 1991. Growth responses of corn (Zea mays L.) to interminating of soil water dificits. Fild Crops Abstracts. Pp: 237.

27. Najafy, M. 2013. The role of amino acids in organic farming. http://www.talfighdaneh.ir/News/post-23550.

28. Pagter, M., Bragato, C., and Brix, H. 2005. Tolerance and physiological responses of phragmites australis to water deficit. Aquatic Botany 81: 285-299.

29. Rahnama, H., and Ebrahimzadeh, H. 2004. The effect of NaCl on proline accumulation in potato seedlings and calli. Physiologiae Plantarum 26 (3): 263-270.

30. Refsum H., Ueland, P.M, Nygård, O., and Vollset, S.E. 1998. Homocysteine and Cardiovascular Disease. Annual Review of Medicine 49: 31-62.

31. Sabaghpour, S.H. 2004. Present status and future prospects of food legume in Iran. In Gowda, C.L.L and Pande, F. (Eds.). Role of legumes in crop diversification and poverty reduction in Asia. The International Crops Research Institute for the Semi-Arid-Tropics. Pp 75-83.

32. Sajedi, N.A., and Rjay, F. 2011. Application of mycorrhizal inoculation on the uptake of zinc and micronutrients in maize. Journal of Soil Research 25: 83-92. (In Persian).

33. Samen, M., Sepehry A., and Ahmadvand, G., 2011. Dry matter accumulation of metabolites produced in six chickpea genotypes under different levels of soil moisture adjustment. Iranian Journal of Biology 24(3): 373-389. (In Persian).

34. Sheykhbagloo1, N., Hassanzadeh, A., Gorttapeh, M., Baghestani, M., and Zand, B. 2009. Study the effect of zinc foliar application on the quantitative and qualitative yield of grain corn under water stress. Electronic Journal of Crop Production 2(2): 59-74. (In Persian).

35. Sood, S., and Nagar, P.K. 2008. Post-harvest alteration in polyamins and ethylene in two diverse rose species. Acta Physiology Plant 30(21): 243-248.

36. Syosemarde, A., Ahmady, A., and Postiny, K. 2009. Stomatal and non-stomatal factors controlling photosynthesis and its relation with drought resistance in wheat. Journal of Agricultural Sciences 35: 93-106. (In Persian).

37. Szabados, L., and Savoure, A. 2009. Proline: a multifunctional amino acid. Trends in Plant Science 15(2): 89-97.

38. Tejo, P.A., and Santos Diaz, M. 1987. Nodule and leaf nitrate reductase and nitrogen fixation in Medicago sativa L. under water stress. Plant Physiology 69: 479-482.

39. Thalooth, A.T., Badr, N.M., and Mohamed, M.H. 2005. Effect of foliar spraying with Zn and different levels of phosphatic fertilizer on growth and yield of sunflower plants grown under saline conditions. Egyptian Journal of Agronomy 27: 11-22.

40. Verbruggen, N., and Hermans, C. 2008. Proline accumulation in plants: a review. Amino Acids 35: 753 -759.

41. Vogt, W. 1995. Oxidation of methionine residues in proteins: Tools, targets, and reversal. Free Radical Biology and Medicine 18: 93-105.

42. Zhang, J., Nguyen, H.T., and Blum, A. 1999. Genetic analysis of osmotic adjustment in crop plants. Journal of Experimental Botany 50: 291-302.