Effect of Salicylic acid and Selenium application at different growth stages on some physiological traits of chickpea under rainfed conditions

Document Type : Original Articles

Authors

• Mojtaba Norouzi ¹
• Nourali Sajedi ²

¹ Ministry of Agriculture-Jahad, Arak, Iran

² Department of Agrotechnology, Arak Branch, Islamic Azad University, Arak, Iran

10.22067/ijpr.v10i2.65944

Abstract

Introduction
 Chickpea (Cicer arietinum L.) is one of the important pulse crops in the world. Also it is valued through high protein content in seeds (23.5-28.9%). In Iran, chickpea produced under arid and semi-arid zones and usually chickpea plants during flowering and maturity stages are exposed to increasing water deficit stress. Different methods are existed in agriculture to increase plants tolerance to abiotic and biotic stresses. Seed priming and foliar application by amino acids, proline, glycinebetaine, Salicylic acid as well as zinc, Selenium and Silicon have been considered in recent years. Selenium is not an essential nutrient for plants. Reports showed that selenium could protect the plants under abiotic stresses such as salinity, high temperature and drought. Selenium protected the plants from abiotic stresses by participate in several physiological and biochemical process such as water condition retain, increasing plant pigments and photosynthesis apparatus regulation. Salicylic acid (SA) is a plant-produced nature phenolic compound which plays an important role in the abiotic stresses tolerance. Growth and production of crops will affect by SA application. Several studies showed that salicylic acid application increased the plant tolerant to drought stress. Increasing proteins, photosynthesis pigments and sugars indicated on the role of salicylic acid in increasing the plant resistance to stress. Therefore, this experiment carried out to study the influence of foliar application of salicylic acid and selenium at different growth stages on some physiological traits of chickpea under rainfed condition.
 
Material & Methods
To evaluate the response of some physiological traits of chickpea at different growth stages to foliar application of salicylic acid and selenium under rain fed condition, an experiment was conducted as split plot based on Randomized Complete Block Design with four replications during 2013-2014 cropping season. Experimental factors were salicylic acid in three levels (0,100 and 200 mg L^-1) and foliar selenium spraying in two levels (0 and 18 gr ha^-1) that applicated in different growth stages (vegetative, reproductive growth and vegetative growth along with reproductive). According to the soil analysis results, 50 kg ha^-1 urea and 40 kg ha^-1 triple superphosphate fertilizers were applied at planting time. Each experimental plot included 30 cm distanced rows with 10 cm spacing between plants in rows. The chickpea cultivar was ‘Azad’. The seeds were sown at 6 cm depth in end of February 2013. Physiological traits measured from three replications. Relative water content was determined from five leaflet from leaves at flowering stage that were prepared immediately and weighed to measure fresh leaf weight in the laboratory at 25°C. Then, leaflet were placed in distilled water for 24 h until completely saturated. At the end of this stage, leaflet were dried with dry paper towels and reweighed. Samples were placed in the oven for 48 h at 72°C until dried and then weight of the dried leaves was recorded. Relative water content was calculated using the following relation. where RWC is the relative water content, Wf is the leaf fresh weight, Wd is the leaf dry weight and Ws is the leaf saturated weight. Photosynthetic pigments were calculated by the method presented by Arnon (1949). Samples collected from five leaflet, 0.5 gr leaves were ground in 80% acetone for determination and absorbance of the resulting extracts, recorded at 480, 510, 645 and 663 nm with a spectrophotometer. Biological and seed yield harvested from 2 m² of middle in each plots. Data were subjected to analysis of variance using SAS program. Means were compared using the Least Significant Diffrence test (LSD) Multiple Range Test at 5% probablity level.
 
Results & Discussion
Results showed that the highest leaf relative water content observed from foliar application of selenium and salicylic acid at vegetative along with reproductive stages. Salicylic acid appli­cation at the rate of 100 and 200 mg L^-1 and selenium at the rate of 18 gr ha^-1 increased the leaf relative water content by 18, 42.7 and 31%, respectively as compared with control. There were reports that, application of salicylic acid improved vegetative growth rate, chlorophyll a, b and total chlorophyll, leaf relative water content and resistance to water deficit in lawn. The highest ion leakage (82.57%) and the lowest (75.86%) recorded from treatments of without application of selenium and foliar application of 18 gr ha^-1 selenium, respectively. Foliar application of 100 and 200 mg L^-1 salicylic acid along with 18 gr ha^-1 selenium increased chlorophyll a content compared with control, significantly. Foliar application of 100, 200 mg L^-1 salicylic acid and 18 gr ha^-1 selenium alone increased chlorophyll b content by 29.4%, 32.3% and 26.4% as compared with control, respectively. The highest carotenoids content belonged to foliar application of 200 mg L^-1 salicylic acid. There were reports that application of selenium decreased chloroplast damage and help to retain of photosynthesis pigments. Foliar application of 100 and 200 mg L^-1 salicylic acid along with 18 gr ha^-1 selenium increased productivity index at different growth stages as compared with control. The treatments interaction effects showed that the maximum biological yields (1528.86 and 1628.40 kg ha^-1) obtained from treatment of 100 and 200 mg L^-1 salicylic acid along with 18 gr ha^-1 selenium at different growth stages, respectively. The results showed that foliar application of salicylic acid and selenium could be increased chickpea seed yield.
 
Conclusion
The positive role of application of salicylic acid and selenium under rainfed condition exerted by the effects on physiological process such as water retain in plant, membrane stability retain and improve of photosynthesis pigments. Therefore, foliar application of 100 mg L^-1 salicylic acid and along with 18 gr ha^-1 selenium at vegetative growth along with reproductive growth could obtain optimum yield.

Keywords

• Chickpea
• Ion leakage
• Plant pigments
• Relative water content
• Sodium selenite

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References

Abul-Soud, M.A., and Abd-Elrahman, Sh.H. 2016. Foliar selenium application to improve the tolerance of eggplant grown under salt stress conditions. International Journal of Plant and Soil Science 9(1): 1-10.

2. Arnon, D.I. 1949. Copper enzymes in isolated chloroplast oxidase in Beta vulgaris. Plant Physiology 24(1): 1-15.

3. Balal, R.M., Shahid, M.A., Javaid, M.M., Iqbal, Z., Anjum, M.A., Garcia- Sanchez, F., and Marttson, N.S. 2016. The role of selenium in amelioration of heat-induced oxidative damage in cucumber under high temperature stress. Acta Physiologiae Plantarum 38(158): 1-14.

4. Broadley, R., Martin, F., and John, A. 2010. Selenium biofortification of high yielding winter wheat (Triticum aestivum L.) by liquid or granular Se fertilization. Plant and Soil 332: 5-18.

5. Cao, F., Cai, Y., Cheng, W.D., Zhang, G.P., and Wu, F.B. 2011. Modulation of exogenous glutathione in phytochelatins and photosynthetic performance against Cd stress in the two rice genotypes differing in Cd tolerance. Biology Trace Elemental Research 143(2): 1159-1173.

6. Chilimba, A.D., Young, S.D., Black, C.R., Meacham, M.C., Lammel, J., and Broadley, M.R. 2012. Agronomic biofortification of maize with selenium in Malawi. Field Crops Research 125: 118-128.

7. Coronado, M.A.G., Lopez, C.T., and Saavedra, A.L. 1998. Effects of salicylic acid on the growth of roots and shoots in soybean. Plant Physiology and Biochemistry 36(8): 563-565.

8. Djanaguiraman, M., Devi, D.D. Shanker, A.K. Sheeba J.A., and Bangarusamy, U. 2005. Selenium-an antioxidative protectant in soybean during senescence. Plant and Soil 272: 77-86.

9. Epstein, E. 2009. Silicon: its manifold roles in plants. Annals Applied Biology 155(2): 155-160.

10. Fariduddin, Q., Hayat S., and Ahmad A. 2003. Salicylic acid influ‌ences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity, and seed yield in Brassica juncea. Photo-synthetica 41: 281-284.

11. Filek, M., Gzyl-Malcher, B., Zembala, M., Bednarska, E.,Laggner, P., and Kriechbaum, M. 2010. Effect of selenium on characteristics of rape chloroplasts modified by cadmium. Plant Physiology 167(1): 28-33.

12. Hadi, M.R., and Balali, G.R. 2010. The effect of salicylic acid on the reduction of rizoctoni damage in the tubers of marfona potato cultvar. American-Eurasian Journal Agricultural and Environment Science 7(4): 492-496.

13. Han-Wens, S., Jing, H., Shu-Xuan, L., and Wei-Jun. K. 2010. Protective role of selenium on garlic growth under cadmium stress. Communications in Soil Science and Plant Analysis 41(10): 1195-1204.

14. Hayat, Q., Hayat, S., Irfan, M., and Ahmad, A. 2010. Effect of exog‌enous salicylic acid under changing environment: A review. Environmental and Experimental Botany 68(1): 14-25.

15. Hussain, M., Malik, M.A., Farooq, M., Ashraf, M.Y., and Cheema, M.A. 2008. Improving drought tolerance by exogenous application of glycinebetaine and salicylic acid in sunflower. Journal of Agronomy and Crop Science 194(3): 193-199.

16. Jabari, H., Akbari, GH.A., Daneshyan, J., Allahdadi, I., and Shahbazian, N. 2007. Effects of water deficit stress on agronomic characteristics of sunflower hybrids. Journal of Agriculture 9(1): 13-22. (In Persian with English Summary).

17. Jami Moieni, M., Borhani, N., and Armin, M. 2016. Effect priming by salicylic acid on seed germination and seedling growth of pea by allelopathy of Datura stramonium L. Journal of Seed Research 21(4): 47-56. (In Persian with English Summary).

18. Keshavarz, H., and Modarres Sanavy, S.A.M. 2014. Effect of salicylic acid on chlorophyll, some growth characteristics and yield of two canola varieties. Journal Crop Production 7(4): 167-178. (In Persian with English Summary).

19. Khan, W., Prithiviraj, B., and Smith, D.L. 2003. Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of Plant Physiology 160(5): 485-492.

20. Lutts, S., Kint, J.M., and Bouharmont, J. 1996. NaCl-induced senescence in leavesof rice (Oriza sativa L.) cultivars differing in salinity resistance. Annals of Botany 78(3): 389-398.

21. Mafakheri, A., Siosemardeh, A., Bahramnejad, B., Struik, P.C., and Sohrabi, Y. 2011. Effect of drought stress and subsequent recovery on protein, carbohydrate contents, catalase and peroxidase activities in three chickpea (Cicer arietinum L.) cultivars. Australian Journal of Crop Science 5(10): 1255-1260.

22. Malik, J.A., Goel, S., Kaur, N., Sharma, S., Singh, I., and Nayyar, H. 2012. Selenium antagonizes the toxic effects of arsenic on mungbean (Phaseolus aureus Roxb) plant by restricting its uptake and enhancing the antioxidative and detoxification mechanisms. Environmental and Experimental Botany 77: 242-248.

23. Nawaz, F., Naeem, M., Ashraf, M.Y., Tahir M.N., Salahuddin M., Shabbir, R.N., and Aslam, M. 2016. Selenium supplementation affects physiological and biochemical processes to improve fodder yield and quality of maize (Zea mays L.) under water deficit conditions. Frontiers in Plant Science 7: 1-13.

24. Nawaz, F., Ashraf, M.Y., Ahmad, R., Waraich, E.A., Shabbir, R.N., and Bukhari, M.A. 2015. Supplemental selenium improves wheat grain yield and quality through alteration sinbiochemical processes under normal and water deficit conditions. Food Chemistry 175: 350-357.

25. Patel, P.K., Hemantaranjan, A., Sarma, B.K., and Singh, R. 2011. Growth and antioxidant system under drought stress in chickpea (Cicer arietinum L.) as sustained by salicylic acid. Journal of Stress Physiology and Biochemistry 7(4): 130-144.

26. Popova, L., Ananieva, E., Hristova, V., Chistov, K., Georgieva, K., Alexieva, V., and Stoinova, Zh. 2003. Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulgarian journal of Plant Physiology (Special Issue): 133-152.

27. Ramezannezhad, R., Lahouti, M., and Ganjali, A. 2013. Effect of salicylic acid on physiological and biochemical parameters on resistant and sensitive chickpea (Cicer arietinum L.) genotypes under drought stress. Plant Eco-Physiology 5(12): 24-37.

28. Rezazadeh, S. 2011. Effect of Salicylic Acid on Agronomic Traits, Yield Components and Nitrogen Biological Fixation in Pea Plant under Drought Stress Condition. Thesis of MSc. in Seed Science and Technology. University of Birjand, Iran. 78p. (In Persian with English Summary).

29. Rodrigo, S., Santamaria, O., Lopez-Bellido, F.J., and Poblaciones, M.J. 2013. Agronomic selenium biofortification of two-rowed barley under Mediterranean conditions. Plant, Soil and Environment 59(3): 115-120.

30. Talebi, R., Ensafi, M.H., Baghebani, N., Karami, E., and Mohammadi, K.H. 2013. Physiological responses of chickpea (Cicer arietinum) genotypes to drought stress. Environmental and Experimental Biology 11: 9-15.

31. Turner, N.C. 1981. Techniques and experimental approaches for the measurement of plant water status. Plant and Soil 58(1-3): 336-339.

32. Vaisnad, Sh., and Talebi, R. 2015. Salicylic acid-enhanced morphological and physiological responses in chickpea (Cicer arietinum) under water deficit stress. Environmental and Experimental Biology 13: 109-115.

33. Wang, Y.D., Wang, X., and Wong, Y.S. 2013. Generation of selenium-enriched rice with enhanced grain yield, selenium content and bioavailability through fertilization with selenite. Food Chemistry 141(3): 2385-2393.

34. Xue, T.L., Hartikainen, H., and Piironen, V. 2001. Antioxidative and growth-promoting effects of selenium on senescing lettuce. Plant and Soil 237: 55-61.

35. Zahedi, H., Shirani Rad, A.H., and Tohidi Moghadam, H.R. 2012. Effect of zeolite and selenium foliar application of on growth, production and some physiological attributes of three canola (Brassica napus L.) cultivar subjected to drought stress. Revista Cientifica UDO Agricola 12(1): 135-142.