Identification of plant parasitic nematodes of bean fields in Markazi province

Document Type : Original Articles

Authors

1 Plant Protection Research Department, Markazi Agricultural and Natural Resources Research and Education Center, AREEO, Arak, Iran

2 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Introduction
Regarding the importance of bean production in supplying of plant based protein for human being societies and its area under cultivation in Iran, identification and management of pests and diseases associated to this crop are important. Plant parasitic nematodes are one of the biotic agents that adversely affect the production of bean crops. In order to manage these pathogens, their identification and distribution is necessary. Plant parasitic nematodes damaging bean are widely reported through the world. In Florida, Belonolaimus longicaudatus ,Paratrichodorus christei, Dolichodorus heterocephalus and Hoplolaimus spp. are reported from bean cultivated field while causing severe damage (Rhoades, 1964, 1974). In Michigan Pratylenchus penetrans was the most common root lesion nematode. In fact Pratylenchus spp. have wide host ranges and are pathogenic to many important crops viz Phaseolus vulgaris. There are a few reports across Iran regarding the plant parasitic nematodes of bean. In a study of plant parasitic nematodes of pulse crops in southern Balochistan, Pratylenchus neglectus and Meloidogyne javanica were reported from bean fields. Five species Aphelenchoides cyrtus, Ditylenchus medicaginis, Merlinius brevidens, D. parvus and Paratylenchus coronatus with 37.6, 30.8, 24.6, 20.5 and 19.1 percent frequency, respectively, were the most common nematode species in the legume fields of Lorestan province. Although Markazi province is one of the poles of bean-growing in Iran but there is a few information about plant parasitic nematodes on bean. The aim of this study was to identify the plant parasitic nematodes associated with bean, determination their population density and geographical distribution.
 
Materials & Methods
In order to determine the plant parasitic nematodes 174 root and soil samples were collected from bean fields in Khomein, Shazand and Arak cities during 2012-2013. The samples were analyzed and the population density of the extracted nematodes was counted at genus level using counting slide. Then the obtained nematodes were fixed and transferred to hydrated glycerol, the nematodes were mounted on microscopic slides and identified at species level, based on morphometrical and morphological characters. Cyst nematodes were isolated using Fenwick can technique. After analyzing the samples, infested areas were identified and frequency based on the number of species compared to the total sample were determined.
 
Results & Discussion
In this study, 22 species belonging to 18 genera of suborder Tylenchina, infraorder Tylenchomorpha were identified. Ditylenchus destructor, Ditylenchus sp., Aphelenchoides sp. and family of Pratylenchidae including P. neglectus, Zygotylenchus guevarai and Pratylenchoides ritteri from family Merliniidae with 100, 58 and 95 percent frequency respectively, were the most common nematode species in the bean fields of Markazi province. Subfamily of Merliniinae the genera of Merlinius, Scutylenchus, Tylenchorynchus and Amplimerlinius with 56 percent frequency, were found in Khomein and Shazand bean fields. The family of Hoplolaimidae was detected in Khomein and Shazand bean fields with 39 percent frequency. Among the cyst forming nematodes in addition to Heterodera filipjevi which was found in bean fields rotated by wheat, H. goettingianawas found in a bean field in Ghasemabad, Arak, this species in known as pea cyst nematode. H. goettingiana had already been reported from two areas in Dorud region (Lorestan province). This species was found in some wheat fields that bean was one of the crops in rotation. According to the occurrence of this species in bean field in Markazi province which borders Lorestan Province, it seems that it is distributed in pea and bean fields in two provinces. Some nematodes identified in this study are important as plant parasiting and damaging for bean. P. neglectus with 93, 42 and 22 percent frequency respectively detected in the bean fields of Khomein, Arak and Shazand. Root lesion nematodes were reported as the most common nematodes on bean and other legumes in Italy, North africa and Middle East. Elliott & Bird (1985) reported damage of root lesion nematodes in bean fields among 10 to 80 percent.Therefore, it is necessary to research on the importance and evaluation the damage caused by this species. Moreover due to the abundance of Ditylenchus in bean fields of Markazi province, it needs intensive research as far as crop loss aspects are concerned.
 
Conclusion
In the present study, among identified nematodes, family of Pratylenchidae was the most important plant parasitic nematode with 58 percent frequency. Also genus of Ditylenchus spp. detected in all of bean fields of Markazi province.

Keywords


  1. Abawi, G.S., Crosier, D.C., and Cobb, A.C. Root rot of snap beans in NewYork. NewYork Food and Life Sciences Bulletin 110:1-7.
  2. Ahmadi, S., Mahdikhani Moghaddam, E., and Baghaee Ravari, S. 2014. Identification of plant parasitic nematode collected from pulse fields in Northern Khorasan province. Iranian journal of Pulses Research 5(2): 111-118. (In Persian with English Summary).
  3. Ahmadian Yazdi, A., and Zohoor Paralak, E. 2007. Survey of root knot nematodes of legume fields in Khorasan provinces (Abstract). In: Abstract Book of the 2nd National Pulse Crops Symposium of Iran, Tehran, p: (In Persian).
  4. Azizi, K., and Karegar, A. 2014. Plant parasitic nematodes of legume fields and their distribution in Lorestan province, Iran. Iranian Journal of Plant Pathology 50(3):219-235. (In Persian with English Summary).
  5. Back, M.A., Haydock, P.P.J., and Jenkinson, P. 2002. Disease complexes involving plant parasitic nematodes and soilborne pathogens. Plant Pathology 51: 683-697.
  6. Bafokuzara, N.D. 1996. Incidence of different nematodes on vegetable and fruit crops and preliminary assessment of yield loss due to Meloidogyne species in Uganda. Nematologia Brasileira 20(1): 32-43.
  7. Barooti, S., Kheiri, A., Tanha-Maafi, Z., and Norozi, R. 2000. Study on plant parasitic nematode fauna in Lorestan province(Abstract). In: Abstract Book of the 14th Iranian Plant Protection Congress, Isfahan, Iran, p: (In Persian with English Summary).
  8. Barooti, S., Tanha-Maafi, Z., Kheiri, A., and Golmohammad Zadeh Khiabani, N. 2002. Plant parasitic nematodes fauna from south of Baloochestan area in Iran(Abstract). In: Abstract Book of the 15th Iranian Plant Protection Congress, Kermanshah, Iran, p: (In Persian with English Summary).
  9. Coolen, W.A., and D′herde, J. 1972. A Method for the Quantitative Extraction of Nematodes from Plant T State Agricultural Research Center. Ghent, Belgium.
  10. De Grisse, A.T. 1969. Redescription ou modification de quelques techniques utiliseesdans L’etude des Nematodes Phytoparasitaires. Meded. Rijksfaculteit der Landbouwe-tenschappen Gent 34:351-369.
  11. Di Vito, M., Greco, N., Halila, H.M., Mabsoute, L., Labdi, M., and Beniwal, S.P.S. 1994. Nematodes of cool-season food legumes in North Africa. Nematologia Mediterranea 22:3-10.
  12. Elliott, A.P. 1980. Ecology of Pratylenchus penetrans ssociated with navy beans (Phaseolus vulgaris). Ph.D. Dissertation, Department of Entomology, Michigan State University.
  13. Elliott, A.P., and Bird, G.W. 1985. Phathogenicity of Pratylenchus penetrans to navybean (Phaseolus vulgaris). Journal of Nematology 17(2): 81-85.
  14. Fenwick, D.W. 1940. Methods for the recovery and counting of cysts of Heterodera schachtii from soil. Journal of Helminthology18: 155-
  15. Franc, G.D., Beaupre, C.M.S., Gray, F.A., and Hall, R.D. 1996. Nematode angular leaf spot of dry bean in Wyoming. Plant Disease 80:476-477.
  16. Ghayedi, S. 2011. Identification of plant parasitic nematodes of bean in Boyer Ahmad region. M.Sc. Thesis. University of Yasouj, Iran. (In Persian with English Summary).
  17. Greco, N., and Di Vito, M. 1994. Nematodes of food legumes in the Mediterranean Basin. Eppo Bulletin 24: 393-398.
  18. Greco, N., Di Vito, M., and Saxena, M.C. 1992. Plant parasitic nematodes of cool seasonfood legumes in Syria. Nematologia Mediterranea 20:37-46.
  19. Hutton, D.G., Wilkinson, R.E., and Mai, W.F. 1973. Effect of two plant parasitic nematodes on Fusarium root rot of beans. Phytopathology 63(6):749-751.
  20. Jenkins, W.R. 1964. A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Diseases 48:
  21. Kimenju, J.W., Karanja, N.K., and Macharia, I. 1999. Plant parasitic nematodes associated with common bean in Kenya and the effect of Meloidogyne infection on bean nodulation. African Crop Science Journal 7(4): 503-510.
  22. Mc Sorley, R. 1979. Damage to snap beans as a function of population gradients ofMeloidogyne incognita in the field. Journal of Nematology 11: 308. (Abstract).
  23. Mc Sorley, R. 1980. Effect of Rotylenchulus reniformis on snap bean and methods for control by oxamyl. Nematropica 10(2): 89-95.
  24. Ministry of Agriculture J 2015. Agricultural Statistics, Crop Year 2011-2012.Available at websitehttp://www.maj.ir/Portal/File/ShowFile.aspx? (In Persian).
  25. Rhoades, H.L. 1964. Effect of Crotalaria spectabilis and Sesbania exaltata on plant nematode populations and subsequent yield of snap beans and cabbage. Proceedings of the Florida State Horticultural Society 77: 233-237.
  26. Rhoades, H.L. 1974. Comparison of two methods of applying granular nematicides for control of sting nematodes on snap beans, sweet corn and field corn. Soil Crop Science Society Florida Proceedings 33:77-80.
  27. Sasser, J.N., and Freckman, D.W. 1987. A World Perspective on Nematology: The Role ofthe Sp. 7-14. In: J.A. Veech and D.W. Dickson (Eds.). Vistas on Nematology.Society of Nematologists, Hyattsville, Maryland. 509p.
  28. Schwartz, H.F., Gent, D.H., Franc, G.D., and Harveson, R.M. 2007. Dry Bean Root K High Plant IPM Guide, University of Wyoming.
  29. Sharma, R., and Sharma, S.B. 2002. Nematode Diseases of Pulses and their Control. In: M. Mashkoor Alam and N.Sharma (Eds.). Nematode Control in Crops. IBDC Publishing, India, p. 95-117.
  30. Sikora, E.J., Kemble, J.M.,and Bauske, E.M. 2004. Plant disease notes: Root knot nematode on snap and lima beans. Alabama Cooperative Extension System. Available at website aces.edu/pubs/docs/A/ANR-1010
  31. Tanha-Maafi, Z., Ebrahimi, N., Mazdasht, G., Ghalandar, M., Mohammadi Pour, M., Ghasemi, M.T., and Nourafkan, M. 2008. Identification and population density of cereal cyst nematodes and root lesion nematodes in north west, west and central provinces in Iran (Abstract). In: Abstract Book of the 18th Iranian Plant Protection Congress, Hamadan, Iran, 590. (In Persian with English Summary).
  32. Tanha-Maafi, Z., Sturhan, D., Kheiri, A., Geraert, E., Subbotin, S.A., and Moens, M. 2004. Morphology of some cyst-forming nematodes from Iran. Russian Journal of Nematology 12: 59-78.
  33. Waddill, V.H., Mc Sorley, R., and Pohronezny, K. 1981. Field monitoring: basis forintegrated management of pests on snap beans. Journal of Tropical Agriculture 58:157-169.
  34. Yazdi Samadi, B., and Abdmishani, S. 2001. Breeding Field C Tehran University Press, 283p.(In Persian).
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