Study of nano particle TiO2 spraying on chlorophyll, yield and yield components of lentil (Lens culinaris Medik)

Document Type : Original Articles

Authors

1 University of Tehran

2 Islamic Azad University, Takestan Branch, Iran

3 Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran

Abstract

Introduction
A particle in which at least one of the dimensions does not exceed 100 nm is classified as a nanoparticle. Nanoparticles are characterized by high ratio of surface area to volume or weight, what strongly influences physical and chemical properties of Nano sized materials. Nano products find applications in many fields of daily life. Lentil is one of the major legumes crops in all over the world including Iran. It is a cheap source of high quality protein in the diets of millions in developing countries, who cannot afford animal protein for balanced nutrition and also rich in important vitamins, minerals, soluble and insoluble dietary fiber.

Material & Methods
To study the effect of nanoparticle Tio2 spray on some agronomic traits of lentil, a factorial experiment was carried out based on RCBD with four replications in Zanjan University on 2011. Treatments included six levels of nanoparticle Tio2 solution spray (control, 0.01%, 0.02%, 0.03%, 0.04% and 0.05%) and two times of spraying (flowering and 60% steaming stage).

Results & Discussion
Results showed spray concentration × application time interaction on grain and biological yield, number of pod per plant, number of grain per pod, 100 grain weight, plant height, number pod hollow and chlorophyll b and harvest index was significant. But spray concentration × application time interaction on chlorophyll a was not significant. Mean comparison of the traits showed that the highest amount of chlorophyll b was obtained with applying 0.01% nanoparticle Tio2 solution at both stage and the lowest obtained with applying 0.01% nanoparticle Tio2 solution at 60% steaming stage. Mean comparison of the traits showed that the highest amount of plant height was obtained with applying 0.01% nanoparticle Tio2 solution at 60% steaming stage and the lowest obtained with applying 0.05% nanoparticle Tio2 solution at 60% steaming stage. Mean comparison of the traits showed that the highest amount of grain yield was obtained with applying 0.02% nanoparticle Tio2 solution at 60% steaming stage. However, with applying 0.01% nanoparticle Tio2 this stage there were not significant and the lowest obtained with applying 0.02% nanoparticle Tio2 solution at flowering stage. Mean comparison of the traits showed that the highest amount of biological yield was obtained with applying 0.02% nanoparticle Tio2 solution at 60% steaming stage and the lowest was obtained with applying 0.02% nanoparticle Tio2 solution at flowering stage. Mean comparison of the traits showed that the highest amount of harvest index was obtained with applying 0.05% nanoparticle Tio2 solution at 60% steaming stage and the lowest was obtained with applying 0.04% nanoparticle Tio2 solution at 60% steaming stage. Mean comparison of the traits showed that the highest amount of pod per plant was obtained with applying 0.01% nanoparticle Tio2 solution at 60% steaming stage and the lowest was obtained with applying 0.04% nanoparticle Tio2 solution at flowering stage. The result of mean comparison showed that the highest amount of number of grain per pod was related with applying 0.01% nanoparticle Tio2 solution at 60% steaming stage and the lowest was related with applying 0.04% nanoparticle Tio2 solution at 60% steaming stage. Mean comparison of the traits showed that the highest amount of 100 grain weight was obtained with applying 0.01% nanoparticle Tio2 solution at 60% steaming stage and the lowest was obtained with applying 0.04% nanoparticle Tio2 solution at flowering stage. As well as, the results showed the effect of spray of titanium dioxide nanoparticles and spraying time on chlorophyll a was significant (P ≤ 0.01). Mean comparison of the traits showed that the highest amount chlorophyll a were obtained with applying 0.05% nanoparticle Tio2, the highest amount chlorophyll a were obtained with applying 0.02% nanoparticle Tio2. The spray of nanoparticles titanium at 50% flowering and steaming stage (60%) was the highest and lowest impact on the amount of chlorophyll a.

Conclusion
According to the results of this study, it is concluded that spray with nanoparticles increased quantity and quality of lentil. But, the use of nanoparticles according to the aspect of environmental safety is very important.

Keywords


1. Abid, H., Chadhary, M.R., Wajid, A., Ahmad, A., Ibrahim, M.R.M., and Goheer, A. R. 2004. Influence of water stress on growth, yield and radiation use efficiency of various wheat cultivars. International Journal Agriculture Biology 6(6): 1074-1079.
2. Ashraf, M., Azim, Y.A.R., Khan, A.H., and Ala, S. A. 1994. Effect of water stress on total phenols, peroxidase activity and chlorophyll content in wheat (Triticum aestivum L.). Acta Physiologia Plantarum 16: 185-191.
3. Chirstianse, I., and Graham, P.H. 2002. Variation in di-nitrogen fixation among Andean bean (Phaseolus vulgaris L.) genotypes grown at low and high levels phosphorus supply. Field Crops Research 73: 133-143.
4. Herzog, H. 1986. Source and sink during the reproductive period of wheat. Scientific publishers, Berlin and Hamburg.
5. Jagtap, V., Bhargara, S., Streb, P., and Feierabend, J. 1998. Comparative effect of water, heat and light stress on photosynthetic reaction in Sorghum biocolor ( L.) Moench. Journal Expend Botany 49: 1715- 1721.
6. Khayyam Nekoei, S., Sharifnasab, M., Ahmadisome, H., Barkhivar, K., and Momeni, M. 2009. A review of nanotech in agricultural-Jehad ministry. Second Edition. Publication of Agricultural Education 23: 45-49 (In Persian).
7. Klushreshtha, S., Mishra, D.P., and Gupta, R.K. 1987. Changes in contents of chlorophyll, proteins and lipids in whole chloroplasts and chloroplast membrane fraction at different leaf water potentials in drought resistant and sensitive genotypes of wheat. Photosynthet 21: 65-70.
8. Kumudini, S. 2002. Trials and tribulations. A review of the role of assimilate supply in soybean genetics yield improvement. Field Crop Research 75: 211-222.
9. Lu C.M., Zhang C.Y., Wu J.Q., and Tao, M.X. 2002. Research of the effect of nanometer on germination and growth enhancement of Glycine max and its mechanism, Soybean Science 21: 168-172.
10. Majnoun Hosseini, N., 2008. Grain Legume Production. Jahad Daneshgahi of Tehran University. Tehran, 283p. (In Persian).
11. Mckenzie B.A., and Hill, G.D. 1995. Growth and yield of two chickpea (Cicer arietinum L.) varieties in Canterbury, New Zealand. New Zealand Journal of Crop and Horticultural Science 23: 467- 474.
12. Meidner, H. 1981. Class Expriments in Plant Physiology. British Library Catalogaing in Publication Data. Londan.
13. Moaveni, P., Aliabadi Farahani, H., and Maroufi, K., 2011a. Effect of Tio2 nanoparticles spraying on wheat (Triticum Aestivum L.) under field condition. Advances in Environmental Biology 5(8): 2208-2210.
14. Moaveni, P., Talebi, R., Farahani, H.A., and Maroufi., K. 2011b. Study of TiO2 nano particles spraying effect on the some physiological parameters in Barley (Hordem Vulgare L.). Advances in Environmental Biology 5(7): 1663-1667.
15. Moaveni, P., Valadabadi, S.A., Aliabadi Farahani, H., and Maroufi, K., 2011c. Nanoparticles TiO2 spraying affected on Calendula (Calendula Officinalis L.) under field condition. Advances in Environmental Biology 5(8): 2242-2244.
16. Oweis T., Hachum A., and Pala, M. 2004. Lentil production under supplemental irrigation in a Mediterranean environment. Agricultural Water Management 68: 251-265.
17. Pilbeam, C.J., Hebblewait, P.D., Rickett, H.E., and Nyongesa, T.E. 1991. Effect of plant population density on determinate and indeterminate forms of winter field bean (Vicia faba). Part 1: yield and yield components. Journal Agriculture Science 116: 373-383.
18. Reynolds, G.H. 2002. Forward to the future nanotechnology and regulatory policy. Pacific Research Institute 1-23.
19. Sabaghpour, S., Safikhani, H., Sarker, M., Ghaffari, A., and Ketata, A. H., 2004. Present status andfuture propects of lentil cultivation in Iran. P, 146, Proceeding of 5th European Conference on Grain. 7-11 June, Dijon, France.
20. Sairam, P.K., Deshmukh, P.S., and Shukla, D.S. 1997. Tolerance of drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. Journal Agronomy Crop Science 178: 171- 178.
21. Salehi, M. 2008. Pretreatment effect of nanosilver on germination and seedling growth of wheat under salt stress. Proceeding of First Iranian Congress in Seed Sciences and Technology. Gorgan, Iran. 358 p. Yazdi Sammadi, B. 2008. Waste reduction in wheat production and consumption. Proceeding of 10th Iranian Cong. of Crop Sciences. Karaj, Iran. 519 p.
22. Sandor, D., Istvan, M., Judit, P., Agota, C., Reka, T., and Marta, M. 2006. Effects of drought on photosynthetic parameters and heat stability of PSII in wheat and in Aegilops species originating from dry habitats. Acta Biologica Szegediensis 50(1-2): 11-17.
23. Schutzz, M., and Fangmeier, A. 2001. Growth and yield responses of spring wheat (Triticum aestivum L. CV. Minaret ). Pollution 114: 187- 189.
24. Soltani, A., Khoie, F.R., Ghassemi, K., and Moghaddam, M. 2001. A simulation study of chickpea crop response to limited irrigation in semi-arid environments. Agricultural Water Management 49: 225-237.
25. Tanaka, A., and Tanaka, R. 2006. Chlorophyll metabolism. Plant Biology 9: 248- 255.
26. Zecevic, V., and Knezevic, D. 2005. Variability and components of variance for harvest index in wheat (Triticum aestivum L.) Genetica 37: 173-179.
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