Effect of fish waste, chemical fertilizer and biofertilizer on yield and yield components of bean (Vigna sinensis) and some soil properties

Document Type : Original Articles

Authors

1 Shahrood University

2 Shahrood university

Abstract

Introduction
One of the acute nutritional problems is protein deficiencies of millions humankind under developing countries. Legumes are one of the important sources of protein after cereals. The range of protein content of legumes is between 18 to 23 percent that makes it a good source of protein for low income people (Mohammadzadeh, 2012). Legumes with biological fixation can improve soil fertility which can be in crop rotation that is useful in soil conservation (Parsa and Bagheri, 2008). In sustainable agriculture as legumes expect low nutrient they can be cultivated in low input agriculture. Bean (Vigna unguiculata sp. Sinensis) is one of the valuable crops from point of nutrient view especially in vitamins and proteins (Zia-Ul-Haq et al., 2013). This crop has wide range of compatibility under different climate and it is cultivated in wide range of soil with good management. In Iran this crop is cultivate for a long time. In regions like Garmsar and Varamine and some alike climate it is one of the main crops. Combination of chemical fertilizer and manure can creat suitable environment for plant growth, as manure has suitable effect on soil properties and chemical fertilizers can cause better nutrient availability. Fish waste is one of the organic fertilizers that has been used for a long time, dating back to Egyptian in Egypt and Red Indian in America. This manure is full of nutrient like nitrogen, phosphorus which can be beneficial to farms. The aim of this research was to study the effect of fish waste manure and chemical fertilizers on qualitative and quaititative properties of bean and soil physical and chemical properties.

Materials & Methods
This experiment was conducted in 2012-2013 in greenhouse of agriculture college of Shahrood University. This research was carried out in the form of factorial based on randomize completly design with three replications. Experimental factors were fish waste in three levels: controls (F0), 500 kg ha-1 (F1) and 1000 kg ha-1 (F3). Chemical fertilizers in three levels: control (N0P0K0), half of the local recommendation (N1P1K1, nitrogen 25 kg ha-1, phosphorus 40 kg ha-1, and potassium 35 kg ha-1) and local recommendation (N2P2K2), nitrogen 50 kg ha-1, phosphorus 80 kg ha-1, and potassium 70 kg ha-1. Psodomonas bacteria with two levels: control (B0) and applied (B1). Physical and chemical properties of soil were analysed before conducting the research. Pots were filled with 6 kg soil and treatments were mixed with the soil and ten seeds were sown in each pot. Plant components like plant height, pod in plants, seed number in pods, total dry weight of shoot, pod dry weight and leaf chlorophyll with SPAD 502 were analyesd. Nitrogen was evaluated with kejehldal, phosphorus with Olsan P (1954), Organic C with walkley and Black methods (1934), pH with pH meter, and ECe with EC meter. All data were analyzed for analysis of variance with MSTATC and Excel 2007 and mean comparisons were done using LSD at 5 percent level of significant.


Results & Discussion
Chemical fertilizers had significant effect on plant height at 1% level of significance. However, the effect of fish waste, psodomonas bacteria and their interactions compare to control had increasing effect on plant hight but was not significant. The main effect and interaction of fish waste and psodomonas bacteria were significant at 1% level on pod weight. However, the highest pod weight was observed in the application of chemical fertilizer at N2P2K2. Nevertheless, the application of chemical fertilizers can provide essential elements to plat for better growth. Application of chemical fertilizers had significant effect on plant total shoot weight, other treatments and their interactions did not have significant effect on plant total shoot weight. Chemical fertilizers application at N2P2K2 and fish waste application had significant effects on leaf chlorophyll. Except chemical fertilizers other treatments had significant effects on seed protein percentage at 1% level of significance. The interaction effects of chemical fertilizer and psodomonas bacteria on seed protein percent had increasing effect compared with control. Application of fish waste had significant effect on seed phosphorus at 5% level of significance, but application of chemical fertilizers and its interaction with fish waste had significant effect at 1% level on seed phosphorus, also their effects and their interactions on seed potassium were significant at 1% level, however psodomonas bacteria had no significant effect on seed potassium. Application of chemical fertilizers had significant effect at 5% level on soil EC and fish waste had significant effect at 1% level on soil electrical conductivity. Application of fish waste and psodomonas bacteria had significant decreasing effect on soil pH compare to control. Amoung all treatments, only fish waste had significant effect on soil organic matter. Chemical fertilizer application and its interaction with fish waste and psodomonas bacteria had significant effect on soil nitrogen percent, but this increase was more in chemical fertilizer application. Between all treatments only application of chemical fertilizers had significant effect on soil available potassium.

Conclusion
According to results, application of fish waste and psodomonas bacteria had significant effect on all evaluated properties of plant and soil.

Keywords


1. Abdizadeh, K., Mahdavedamghani, A.H., Sabahi, H., and Sofizadeh, S. 2011. Effect of amount and methods biological fertilizer and fertilizer combination on yield and yield components of seed maize. Electronical Journal of Agricultural Production 4(3): 21-35. (In Persian with English Summary).
2. Ahmadabadi, Z., and Ghajarsepanloo, M. 2012. Effect of organic fertilizer application on some soil physical properties. Journal of Soil and Water Conservation Research 12(2): 99-116. (In Persian with English Summary).
3. Akbarineya, A., Sefidkan, F., Rezaei, M.B., and Shareifi Ashorabadi, A. 2003. Effect of chemical fertilizer, manure and integrated use on yield and Zenian essence composition. Research and Development in Agronomy and Hhorticulture 61: 32-41.
4. Aliehyaei, M., and Behbehanizadeh, A.A. 1994. Soil Chemical Analysis Methods. Technical Journal of Soil and Water Research. Tehran. Iran. (In Persian with English Summary).
5. Arvanitoyannis, I.S., and Kassaveti, A. 2008. Fish industry waste: treatments environmental impacts current and potential uses. International Journal of Food Science & Technology 43: 726-745.
6. Barker, A.V., and Pilbeam, D.J. 2007. Handbook of Plant Nutrition. Printed in the United Stat of America. p: 120-124.
7. Bagheri, A.R., Nezami, A., and Porsa, H. 2006. An analysis to strategy of pulse research in Iran based upon the first national pulse symposium approaches. Iranian Journal of Field Crops Research 4(1): 1-13. (In Persian).
8. Baure, A,. and Black, A.L. 1992. Organic carbon effects on available water. Soil Science Society of America Journal 56: 248-254.
9. Chapman, H.D., and Pratt, P.F. 1961. Methods of Analysis for Soil, Plants and Waters. University of California. Division of Agriculture. Sciences Pp: 309.
10. Ebrahimi, N., Afiuoni, M., Karami, M., and Rezaeinezhad, Y. 2008. Residual effect of organic fertilizer accumulation on nitrogen concentration, phosphorus and potassium in soil and wgeat. Journal of Science and Technology of Agriculture and Natural Resources 46: 803-812. (In Persian with English Summary).
11. El-Tarabily, A.K., Nassar, A.H., Giles, E., Hardy, S.J., and Sivasithamparam, K. 2003. Fish emulsion as a food base for rhizobacteria promoting growth of radish (Raphanus sativus L. var. sativus) in a sandy soil. Plant and Soil 252: 397-411.
12. Hiscox, J.D,. and Israelstam, G.F. 1978. A method for the extraction of chrophyll from leaf tissue without naceration. Canadian Journal of Botany 57: 1332-1334.
13. Illera-Vives, M., Seoane Labandeira, S., and Lopez-Mosquera, M.E. 2013. Production of compost from marine waste: evaluation of the product for use in ecological agriculture. Journal of Applied Phycology (25): 1395-1403.
14. Jones, J. 2001. Laboratory guide for conducting soil tests and plant analysis. Journal of Botany 41(3): 1373-1384.
15. Khoramdel, S., Kochakei, A.L., Naseirimahalatei, M., and Ghorbani, R. 2008. Effect of biological fertilizer on Nigella sativa growth index. Journal of Agricultural Research 6(2): 285-294. (In Persian with English Summary).
16. Marschner, H. 1995. Mineral Nutrition of Higher Plants. 2nd Academic Press. Ltd. London 889 pp.
17. Lee, J. 2010. Effect of application methods of organic fertilizer on growth, soil chemical properties and microbial densities in organic bulb onion production. Scientia Horticulturae 124(3): 299-305.
18. Lema, A., and Degebassa, A. 2013. Comparison of chemical fertilizer, fish offal’s fertilizer and manure applied to tomato and onion. African Journal of Agricultural Research 8(3): 274-278.
19. Mahmmodabadi, M,. Rashidi A.L., and Fekri, M. 2013. Effect of alfalfa remaining, chicken manure and potassium fertilizer on some soil properties and onion yield. Journal of Industry and Agricultural Science 27(2): 452-461. (In Persian with English Summary).
20. Majnon Hosseini, N. 1993. Legumes of Iran. Jahad Daneshgahi. Tehran University Publication. P. 24.
21. Mansorifar, S., Modaressanavei, A.A., and Mohammadi, K. 2010. Effect of water stress and nitrogen on yield and yield components of hybrid maize. Journal of Soil and Water 21(2): 29-45. (In Persian with English Summary).
22. Miransari, M. 2011. Soil microbes and plant fertilization. Microbiol Biotechnol 92: 875-885.
23. Mohammadzadeh, A., Majnonhosseini, N., Moghadam, H., and Akbari, M. 2012. Effect of different dry stress levels and nitrogen on yield and yield components of two red bean verieties. Journal of Agricultural Science of Iran 1(43): 29-38. (In Persian with English Summary).
24. Olsen, S.R., Cole, C.V., Watanabe, F.S., and Dean, L.A. 1954. Estimation of available phosphorus in soils by extraction with sodiu bicarbonate. United States Department of Agriculture Circular 939: 1-19.
25. Parsa, M., and Bagheri, A. 2008. Pulses. Mashhad University. 522. (In Persian).
26. Rahdari, B, 2011. Study on the effect of mineral elements deficiency (nitrogen, phosphorus, potassium, calcium and magnesium) on dry weight, fresh weight, root length, areal part and residual water content onfenugreek (Trigonella foenum-graecum). Journal of Natural Ecosystem of Iran 1: 1-10. (In Persian with English Summary).
27. Schlemmer, M.R., Francis, D.D., Shanahan, J.F., and Schepers, J.S. 2005. Rmotely measuring chlorophyll content in corn leaves with differing nitrogen levels and relative water content. Agronomy Journal 97: 106-112.
28. Toor, R.K., Savage, G.P., and Heeb, A. 2006. Influence of different types of fertilizers on the major antioxidant components of tomatoes. Journal of Food Composition and Analysis 19: 20-27.
29. Walkley, A.J., and Black, I.A. 1934. Estimation of soil organic carbon by the chromic acid titration method. Soil Science 37: 29-38.
30. Zeng, D.Q., Brown, P.H., and Holtz, B.A. 1999. Potassium fertilization and diagnostic, criteria for pistachio trees. Better Crops 83(3): 10-12.
31. Zia-Ul-Haq, M., Ahmad, S., Amarowicz, R., and De Feo, V. 2013. Antioxidant activity of the extracts of some cowpea (Vigna unguiculata (L) Walp.) cultivars commonly consumed in Pakistan. Molecules 18: 2005-2017.
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