Investigation of resistance to chocolate spot (Botrytis fabae sard) in faba bean (Vicia faba L.) in Gorgan region

Document Type : Original Articles

Authors

• Fatemeh Sheikh
• Hamid Dehghani

Tarbiat Modares University, Tehran

10.22067/ijpr.v1393i1.46244

Abstract

Fungal diseases, chocolate spot (Botrytis fabae Sard) is the most destructive diseases of faba bean and causes considerable dramatic damage losses on faba bean yield worldwide. The levels of resistance to chocolate spot were evaluated in 35 faba bean cultivars and one susceptible check (ILB365) and local check (Barkat) at the Gorgan Agricultural Research Station during the 2010 and 2011 cropping seasons. The obtained results showed a significant difference (P< 0.01) among genotypes for yield, yield component and related traits to disease resistance. Barkat, FLIP03-35FB and FLIP03-58FB produced the highest plant height, seed yield and 100 seed weight, respectively. In order to determine genotype reaction to chocolate spot, disease symptoms scored in certain intervals until harvest, then AUDPC, rAUDPC and sAUDPC was calculated. A significant variation among the tested genotypes and their reaction to chocolate spot disease was found. Results of variance, comparing the mean and also cluster analysis revealed that ILB 365, FLIP03-57FB, FLIP03-43FB and FLIP03-59FB where susceptible. Whereas, Icarus, FLIP03-29FB, FLIP03-30FB, FLIP03-34FB and FLIP03-52FB introduced as resistance genotypes to chocolate spot judged by the lowest AUDPC.

Keywords

• Area Under the Disease Progress Curve
• Cluster analysis
• Disease Severity
• yield

---

References

1. Aghajani, M., Razinataj, A., and Mohammadi, H. 2009. Identification and Management Guide for Broad Bean Diseases. Rashad Publishers. 87p. (In Persian)

2. Anastasios S.L., Dimitrios G.R., and Christos, A.D. 2004. Evaluation of faba beans for resistance to sclerotinia stem rot caused by Sclerotinia trifoliorum. Phytoprotection 85: 89-94.

3. Barnett, H.L., and Hunter, B.B. 1998. Illustrated Genera of Imperfect Fungi. Fourth Edition. American Phytopathological Society Press.

4. Berkely, M.G. 1983. Gardners Chronicle. In: P.D. Hebblethwaite (Ed.). The Faba Bean (Vicia faba L.). A Basic for Improvement. Cambridge, UK. 573p.

5. Bouhassan, A., Sadiki, M., and Tivoli, B. 2004. Evaluation of a collection of faba bean (Vicia fabae L.) genotypes originating from the Maghreb for resistance to chocolate Spot (Botrytis fabae) by assessment in the field and laboratory. Euphytica 135: 55-62.

6. Campbell, C.L., and Modden, L.V. 1990. Introduction to Plant Disease Epidemiology. John Willey and Sons, New York, 532p.

7. Fininsa, C., and Yuen, J. 2002. Temporal progression of bean common bacterial blight (Xanthomonas campestries pv. phaseoli). European Journal of Plant Pathology 108: 485-495.

8. Hanounik, S.B., and Maliha, M. 1986. Horizontal and vertical resistance in Vicia faba to chocolate spot caused by Botrytis fabae. Plant Disease 70: 770-773.

9. Hawthorne, W. 2004. Faba bean disease management strategy for southern region. http://www.sardi.sa.gov.au/pdfserve/fieldcrops/publications/advicefactsheets/brochure.pdf

10. Hosni, A.M., Zed, N.A., and Habib, W.F. 1981. Variation within the fungus Botrytis fabae sard. FABIS Newsletter 3: 49-50.

11. Jamali, A.R. 2009. Identification of sources of disease resistance in Chinese faba bean germplasm for incorporation in Australian cultivars. MSc. Thesis. University of Adelaide, South Australia.

12. Jelenic, S., Mitrikeski, P.T., Papes, D., and Jelaska, S. 2000. Agrobacterium-mediated transformation of broad bean (Vicia faba L). Food Technology and Biotechnology 38: 167-172.

13. Juan, L.L. 1993. Research on breeding and germplasm of autumn sown faba bean. Faba Bean Information Services Newsletter 32:3-22.

14. Kamel, A., and Malila, N.F. 1990. Faba bean diseases in Tanisa. FABIS 10: 20-25.

15. Liang, X.Y. 1986. Faba bean diseases in China. FABIS Newsletter, Faba Bean Information Service, ICARDA 15: 49-51.

16. MacLeod, B., and Sweetingham, M. 1999. Faba bean, Chocolate Spot Disease. In: A.W. Australia (Ed.). Farm Note, Perth Australia. Available at Web site http://www.agric.wa.gov.au/ (verified 1 August 2012).

17. Makkouk, K.M., and Hanounik, S.B. 1993. Major faba bean disease with special emphasis on viral disease. pp. 123-137. In: M.C. Saxena, S. Weigand and L. Li-Juan (Eds.). Faba bean Production and Research in China. International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria.

18. Milus, E.A., and Line, R.F. 1986. Gene action for inheritance of durable, high-temperature, adult plant resistances to stripe rust in wheat. Phytopathology 76: 435-441.

19. Moghaddam, M., Mohamadi, S.A., and Aghaee-Sarbarzeh, M. 1995. Introduction to Multivariate Statistical Methods. 281pp. (In Persian).

20. Paull, J.G., and White, P. 2004. Breeding for the future-southern and western regions. In: J. Egan, J. Paull, A. Mayfield and Hawthorne (Eds). Faba and broad beans: Delivering a quality product in to the furture. Tanunda, South Australia. pp. 66-70. (Pulse Australia).

21. .Rhaiemi, A., Cherif, M., Kharrat, M., and Harrabi, M. 2002. New faba bean genotypes resistant to chocolate spot caused by Botrytis fabae. Phytopathol. Mediterr 41: 99-108.

22. Rossi, V. 1999. Effect of host resistance and fungicide sprays against Cercospora leaf Spot in different sugar beet-growing areas of the Mediterranean basin. Phytopathologia Mediterranea 38(2): 465-470.

23. Sahile, S., Chemed, F., Sakhuja, P.K., and Ahmed, S. 2008. Effect of mixed cropping and fungicides on chocolate Spot (Botrytis fabae sard) of faba bean (Vicia faba L.) in Ethiopia. Crop Protection 27: 275-282.

24. Sarparast, R. 2008. A Final Report of the International Faba Bean Chocolate Spot Experiment Nursery. Publishing Seed and Plant Improvement Institute of Karaj. (In Persian).

25. Staats, M., Baarlen, P., and van Kan, J.A.L. 2012. Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity. Molecular Biology and Evolution 22(2).

26. Singh, A., and Rao, M.V. 1989. Area under disease progress curve: its reliability as a measure of slow-rusting resistance. Plant Breeding 103: 319-323.

27. SPSS Inc. 2010. Released IBM SPSS Statistics for Windows, Version 19.0.

28. Stoddard, F.L., Nicholas, A.H., Rubiales, D., Thomas, J., and Villegas-Fernandez, A.M. 2010. Integrated pest management in faba bean. Field Crop Research 115: 308-318.

29. Tivoli, B., Baranger, A.C., Avila, M., Banniza, S., Barbetti, M., Chen, W., Davidson, J., Lindeck, K., Tivoli, B., Berthelem, P., Leguen, J., and Onfroy, C. 1988. A study of the performance of certain Faba bean genotypes in relation to Botrytis fabae and Ascochyta fabae in France. FABIS Newsletter 21: 36-40.

30. Tivoli, B., Baranger, A., Avila, C.M., Banniza, S., Barbetti, M., Chen, W., Davidson, J., Lindeck, K., Kharrat, M., Rubiales, D., Sadiki, M., Sillero, J.C., Sweetingham, M. and Muehlbauer, F.J. 2006. Screening techniques and sources of resistance to foliar diseases caused by major necrotrophic fungi in grain legumes. Euphytica 147: 223-253.

31. Torres, A.M., Roman, B., Avila, C.M., Satovic, Z., Rubiales, D., Sillero, J.C., Cubero, J.I., Ulukan, H., Guler, M., and Keskin, S. 2003. A path analysis some yield and yield components in Faba bean (Vicia faba L.) genotypes. Pakistan Journal of Biological Sciences 6: 1951-1955.

32. Villegas-Fernandez, A.M., Sillero, J.C., and Rubiales, D. 2011. Screening faba bean for chocolate Spot resistance: evaluation methods and effects of age of host tissue and temperature. European Journal of Plant Pathology 132: 443-453.