Evaluation of the effect of chickpea seeds inoculation with rhizobium, arbuscular mycorhiza and like-endo mycorhiza on yield and yield components of chickpea genotypes (Cicer arietinum L.)

Document Type : Original Articles

Authors

• Mohammad Javad Arshadi ¹
• mahdi parsa ²
• amir lakzian ²
• mohammad kafi ²

¹ Ferdowsi

² ferdowsi

10.22067/ijpr.v8i2.47392

Abstract

Introduction
In Iran, among pluses, chickpea has been allocated the most area under cultivation. However, the yield average of water and dry chickpea is 1000 and 500 kg per hectare, respectively and among the producer countries of this product, the yield average is low. One of the factors which could be effective in increasing yield of chickpea is its symbiosis with the mycorrhizal fungi. Mycorrhizal fungi because of stimulating the development of the root system of plants can affect food absorption. In addition to, mycorrhizal fungi specially provide biological phosphorus absorption for plants involved. Among them, the endo myccorihza (or Vesicular Arbuscular Mycorrhiza) that abbreviated VAM, in creation of symbiosis with the roots of chickpea have been more successful. The mycorrhizal fungi and rhizobium bacteria before create symbiosis with host plant, directly affect the overlay in rhizosphere environment of host plant. Studies have shown that the VAMs (which are newly named AMF ) are generally belong to Zigomaycota groups and ecto-myccorihza are mainly to Basidiomaycota. However recently a new species of Basidiomaycota has been identified with name of Piriformospora indica that acts as AMF and is an entophyte fungus. That is why it is called to be like-endo myccorihza. However, the Triplet symbiosis of chickpea, mycorrhiza and rhizobium and also chickpea genotypes response to this symbiosis should be examined.

Materials & Methods
In order to investigate the inolulation of kabuli seeds of chickpea genotypes with arbuscular mycorrhiza and like-endo mycorhiza, an experiment was conducted in 2014, in split plot by arrangement of two - factors with a randomized complete block design and three replications in research field at Faculty of Agriculture, Ferdowsi University of Mashhad. Main plots were consisted of three levels of mycorhiza (arbuscular mycorhiza of Glomus mosseae, like-endo mycorhiza of Piriformospora indica and non - used mycorhiza) and sub plots were consisted of nine genotypes of chickpea: MCC 80, MCC 358, MCC 361, MCC 392, MCC 427, MCC 537, MCC 693, MCC 696 and MCC 950. (These genotypes had good yield potentials and selection and presented in the studies on germplasm from the Institute of Plant Sciences, Ferdowsi University of Mashhad seed bank). All seeds of genotypes were infected to the symbiotic rhizobium bacteria of chickpea.

Results & Discussion
The results indicated that mycorrhiza significantly increased seed and biological yield, harvest index and number of pods per plant. The effect of mycorrhiza was not significant on number of seeds in pod and weight of 1000 seeds. Among the genotypes in this study, the highest seed and biological yield and number of pods per plant was dedicated to genotype MCC 537. Genotypes MCC 80, MCC 537 and MCC 392 showed highest harvest index significantly. The highest weight of 1000 seeds was assigned to the genotype of MCC 392. There was not significant differences between genotypes due to the number of seeds per pod. In the study the interactions effects was observed between mycorrhizal and chickpea genotypes that the highest seed and biological yield belonged to the factor of arbuscular mycorrhiza-MCC 537 genotype and the highest harvest index belonged to the factor of arbuscular mycorrhiza-MCC 80 genotype.

Conclusion
Application of dual inoculation of mycorrhiza and rhizobium could improve the uptake of yield and yield components in chickpea genotypes. Application of like-endo mycorrhiza had not significant effect on seed production and yield components on chickpea. In a general conclusion, among the studied genotypes, MCC 537 and MCC 427 were better than the others.

Keywords

• Arbuscular mycorhiza
• Chickpea
• Like-endo mycorhiza
• Triplet symbiosis

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References

1. Abrishamchi, P., Ganjeali, A., and Sakeni, H. 2012. Evaluation of morphological triats, proline content and antioxidant enzymes activity in chickpea genotype (Cicer arietinum L.) under drought stress. Iranian Journal of Pulses Research 3(2): 17-30. (In Persian with Enlish Summary).

2. Alimadadi, A., Jansuz, M.R., Besharati, H., and Tavakol Afshari, R. 2010. Evaluate the effect of phosphate-solubilizing microorganisms, mycorrhiza and seed priming on nodulation in chickpea. Journal of Soil Research 24(1): 43-51. (In Persian with Enlish Summary).

3. Asadi Rahmani, H., Asgharzadeh, A., khavazi, K., Rejali, F., and Savaghebi, G.R. 2007. Siol Biological Fertility. Publication of Jahad Daneshgahi. 311 pp. (In Persian).

4. Darzi, M.T., Ghalavand, A., and Rejali, F. 2009. Effect of mycorrhiza, vermicompost and phosphate biofertilizer application on flowering, biological yield and root colonization in fennel (Foeniculum vulgare Mill.). Iranian Journal of Crop Sciences 10(1): 88-109. (In Persian with Enlish Summary).

5. Diouf, D., Diop, T.A., and Ndoye, I. 2003. Actinorhizal, mycorhizal and rhizobial symbioses: how much do we know? African Journal of Biotechnology 2(1): 1-7.

6. Ehyaee, H.R. 2010. Foliar application of methanol and irrigation of regims on two chickpea genotypes morphological characters in field and green house. MSc. Thesis of Faculty of Agriculture. Ferdowsi University of Mashhad. (In Persian with Enlish Summary).

7. FAO. 2004. Agricultural Production Year Book. Rome. Italy.

8. Farshadfar, A., and Javadi Niya, J. 2011. Evaluation of chickpea (Cicer arietinum L.) genotypes for drought tolerance. Breeding Seed and Plant Journal 1-27(4): 517-537. (In Persian with Enlish Summary).

9. Ford Denison, R., and Toby Kiers, E. 2011. Life histories of symbiotic rhizobia and mycorrhizal fungi, a review. Current Biology 21(18): 775-785.

10. Ghalavand, A., Mohammadi, Kh., Aghaalikhani, M., Sohrabi, Y., and Heydari, Gh. 2012. Effects of different organic and biological fertilizers on yield and yield components of chickpea (Cicer aritenium L.). Agronomy Journal (Pajouhesh & Sazandegi) 94: 41-49. (In Persian with Enlish Summary).

11. Ganjeali, A., and Bagheri, A.R. 2010. Evaluation of root morphological characteristics of chickpea (Cicer arietinum L.) in response to drought stress. Iranian Journal of Pulses Research 1(2): 101-110. (In Persian with Enlish Summary).

12. Ganjeali, A., Joveynipour, S., Porsa, H., and Bagheri, A.R. 2011b. Sellection for drought tolerance in Kabuli chickpea genotype (Cicer arietinum L.) in Neyshabour region. Iranian Journal of Pulses Research 2(1): 27-38. (In Persian with Enlish Summary).

13. Ganjeali, A., Kafi, M., Bagheri, A.R., and Sharyari, F. 2005. Sellection for drought tolerance in chickpea genotype (Cicer arietinum L.). Iranian Journal of Pulses Research 3(1): 103-122. (In Persian with Enlish Summary).

14. Ganjeali, A., Porsa, H., and Bagheri, A.R. 2011a. Response of yield and morphophysiological characteristics of earliness chickpea genotype (Cicer arietinum L.) under drought stress. Iranian Journal of Pulses Research 2(1): 65-80. (In Persian with Enlish Summary).

15. Gupta, M.L., Prasad, A., Ram, M., and kumar, S. 2002. Effect of the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus fasiculatum on the essential oil yield related characters and nutrient acquisition in the crops of different cultivars of menthol mint (Mentha arvensis) under field conditions. Bioresource Technology 81: 77-79.

16. Harrach, B.D. 2009. Abiotic and biotic stress effects on barley and tobacco plants. Plant Protection Institute of the Hungarian Academy of Sciences in Budapest.

17. IBPGR. ICRISAT and ICARDA. 1993. Description for Chickpea (Cicer arietinum L.). Printed by ICRISAT.

18. Izadi Darbandi, A., and Akram, L. 2012. Investigate the effect of Pyridate, bentazon and Imazethapyr herbicide on growth, nodulation and biological nitrogen fixation in chickpea (Cicer arietinum L.). Iranian Journal of Pulses Research 3(1): 94-105. (In Persian with Enlish Summary).

19. Jamshidi, E., Ghalavand, A., Sephidkon, F., and Mohamadi Galtapeh, E. 2011. Effects of different nutrition systems (organic, chemical, biological and integrated) and fungi Piriformospora indica on yield and concentration of elements in shoot and grain of fennel (Foeniculum valgare mill). Environmental Sciences 8(40): 50-72. (In Persian with Enlish Summary).

20. Kamaei, R. 2014. Effects of plant species and biological, chemical fertilizers and manure on mycorrhiza infectivness under greenhouse conditions. MSc. Thesis of Faculty of Agriculture. Ferdowsi University of Mashhad. (In Persian with Enlish Summary).

21. Kari Dolatabadi, H., and Mohamadi Galtapeh, E. 2010. In vivo biological activity of Piriformospora indica, Sebacina vermifera and Trichoderma spp. against Fusarium wilt of lentil. Plant Protection Journal 2(2): 127-143. (In Persian with Enlish Summary).

22. Koocheki, A., Zand, A., Banayan, M., Rezvani Moghadam, P., Mahdavi Damghani, A., Jami Alahmadi, M., and Vesal, S. 2005. Plant Eco-Physiology. Vol. 2. Publications of Ferdowsi University of Mashhad. (In Persian).

23. Kumar, V., Sahai, V., and Bisaria, V.S. 2011. High-density spore production of Piriformospora indica, a plant growth-promoting endophyte, by optimization of nutritional and cultural parameters. Bioresource Technology 102: 3169-3175.

24. Mansoorifar, S., Shaban, M., Ghobadi, M., and Sabaghpoor, S.H. 2012. Physiological characteristics of chickpea (Cicer arietinum L.) cultivars under drought stress and nitrogen fertilizer as starter. Iranian Journal of Pulses Research 3(1): 101-110. (In Persian with Enlish Summary).

25. Namvar, P. 2014. Evaluation of effects of Piriformospora indica on nitrogen and phosphorus uptake in corn. MSc. Thesis of Faculty of Agriculture. Ferdowsi University of Mashhad. (In Persian with Enlish Summary).

26. Nazami, A., Pouramir, F., Momeni, S., Porsa, H., Ganjeali, A., and Bagheri, A. 2012. Evaluation of a subset of chickpea germplasm collection of the Ferdowsi University of Mashhad Seed Bank, Kabuli type chickpea. Iranian Journal of Pulses Research 3(1): 17-30. (In Persian with Enlish Summary).

27. Rezvami, M., Ardakani, M.R., Rejali, F., Noor Mohamadi, Gh., Zafariyan, F., and Teymuri, S. 2009. The effect of different strains of mycorrhizal fungi on root characteristics and concentrations of phosphorus, potassium, iron, zinc on Alfalfa (Medicagi sativa L.). Journal of Modern Knowledge of Agriculture 5(15): 55-67. (In Persian with Enlish Summary).

28. Sedaghat Khahi, H., Parsa, M., Nezami, A., Bagheri, A., and Porsa, H. 2012. Evaluation of the morphological and phenoological characteristics of cold tolerance chickpea (Cicer arietinum L.) genotype at Entezary sowing in Mashhad condition. Iranian Journal of Pulses Research 3(1): 41-52. (In Persian with Enlish Summary).

29. Shrimant Shridhar, B. 2012. Review: nitrogen fixing microorganisms. International Journal of Microbiological Research 3(1): 46-52.

30. Singh. D.N., Massod Ali, R.I., and Basu, P.S. 2000. Genetic variation in dry matter partitioning in shoot and root influences of chickpea to drought. 3rd International Crop Science Congress 2000. Hamburg Germany.

31. Solaiman, A.R.M., Rabbani, M.G., and Moll, M.N. 2005. Effects of inoculation of Rhizobium and arbuscular mycorrhiza, poultry litter, nitrogen, and phosphorus on growth and yield in chickpea. Korean Journal of Crop Science 50: 256-261.

32. Tadayyon, M.R., and Ghorbaninejad, A.J. 2012. Effect of supplementary irrigation and compost application on morphological triats and yield of two chickpea (Cicer arietinum L.) cultivars. Iranian Journal of Pulses Research 3(2): 31-44. (In Persian with Enlish Summary).

33. Verma, S., Varma, A., Rexer, K., Kost, G., Sarbhoy, A., Bisen, P., Butehorn, B., and Franken, P. 1998. Piriformospora indica, gen: A new root-colonizing fungus. Mycologia 95: 896-903.

34. Zaidi, A., Khan, M.S., and Amil, M. 2003. Interactive effects of rhizotrophic microorganisms on yield and nutrient uptake of chickpea (Cicer arietinum L.). European Journal of Agronomy 19: 15-21.

35. Zare Mehrjerdi, M., Bagheri, A.R., and Vesal, S. 2011. Selective method for low temperature tolerance in chickpea (Cicer arietinum L.). First National Conference of Pulses. (In Persian with Enlish Summary).