بررسی بیان موقت پروتئین نوترکیبCCL21 انسانی در گیاه نخود به روش آگرواینفیلتراسیون

نوع مقاله : مقالات پژوهشی

نویسندگان

• ماریا بیهقی ¹
• عبدالرضا باقری ^{2، 3}
• حسن مرعشی ²
• مجتبی سنکیان ⁴
• افسانه سادات فرساد ⁵

¹ گروه زیست‌شناسی، مؤسسه آموزش عالی کاویان، مشهد، ایران

² گروه بیوتکنولوژی و بِه‌نژادی گیاهان زراعی، دانشکدة کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

³ گروه بقولات پژوهشکدة علوم گیاهی،دانشگاه فردوسی مشهد ، مشهد، ایران

⁴ پژوهشکده بوعلی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

⁵ دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

10.22067/ijpr.v10i1.60228

چکیده

کموکاین CCL21 از جمله کموکاین­هایی است که خاصیت آنتی‌توموری دارد و در ایمونوتراپی بر پایه سیتوکنین‌ها بر علیه سلول­های سرطانی مؤثر می­باشد. با توجه به اهمیت فناوری جدید تولید پروتئین­های نوترکیب در گیاهان، تولید پروتئین CCL21 نیز در گیاه نخود که دارای خواص آنتی‌توموری و ترکیبات معدنی و شیمیایی مناسب است، مورد توجه قرار گرفته است. در مطالعه حاضر از بیان موقت پروتئین CCL21 به روش آگرواینفیلتراسیون، برای تولید این پروتئین نوترکیب در گیاه نخود استفاده شده است. 72‌ساعت بعد از آگرواینفیلتراسیون، نتایج آزمون­های RT-PCR، Real time PCR، دات‌بلات، وسترن‌بلات و الایزا نشان داد که سازه ژنی طراحی شده در برگ­های نخود با میزان بالایی در سطح رونویسی و ترجمه بیان شده است. بنابراین با استفاده از روش آگرواینفیلتراسیون و انتقال سازه بیانی CCL21، نتایج نشان داد که نخود می­تواند گیاه مناسبی جهت تولید پروتئین­های نوترکیب باشد.

کلیدواژه‌ها

• آگرواینفیلتراسیون
• پروتئین نوترکیب
• ژن ccl21
• نخود

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