اثر همزیستی میکوریزایی و سودوموناس بر صفات مورفوفیزیولوژیکی ماش (Vigna radiata (L.) Wilczek) تحت شرایط تنش رطوبتی

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه آزاد اسلامی واحد میانه

چکیده

آزمایشی به‌صورت اسپلیت پلات در قالب طرح بلوک‌های کامل تصادفی با سه تکرار طی سال زراعی94-1395 در مزرعه تحقیقاتی- آموزشی دانشگاه آزاد اسلامی واحد میانه اجرا شد. تیمارهای تنش رطوبتی در سه مرحله شامل: آبیاری معمولی، قطع آبیاری موقع گلدهی و قطع آبیاری موقع غلاف‌بندی و تیمارهای تلقیح شامل: بدون تلقیح، تلقیح با گلوموس موسه، تلقیح با سودوموناس فلورسنت سویه 169و تلقیح توأم گلوموس موسه+سودموناس فلورسنت سویه 169 بود. بر اساس تجزیه واریانس داده ها، اثر تیمارهای تنش رطوبتی براساس اکثر صفات رشدی معنی‌داری بود و از لحاظ نوع تلقیح به غیر از صفات تعداد برگ در بوته و قطر ساقه، از لحاظ سایر صفات اختلاف معنی‌داری مشاهده شد. تنش رطوبتی در مرحله گلدهی بیشترین کاهش در محتوی نسبی آب برگ را منجر گردید. تلقیح گلوموس موسه به ترتیب منجر به افزایش 3/44 و 4/8 درصدی وزن خشک برگ و وزن خشک گیاه نسبت به تیمار شاهد شد. مرحله تشکیل غلاف حساس‌ترین مرحله رشدی گیاه براساس نیاز به آبیاری تعیین گردید. تلقیح توأم سودوموناس فلورسنت سویه 169 و گلوموس موسه به‌طور هم‌افزایی موجب بالاترین وزن خشک ساقه+غلاف، درصد کلونیزاسیون ریشه و درصد نیتروژن دانه شد. بیشترین میزان پروتئین دانه با 5/16 درصد در تیمار قطع آبیاری در مرحله پرشدن غلاف و در تیمار تلقیح توأم گلوموس موسه و سودوموناس فلورسنت سویه 169 مشاهده شد. تلقیح سودوموناس فلورسنت سویه 169 بالاترین پاسخ رشد میکوریزایی را در تیمار قطع آبیاری در مرحله گلدهی نشان داد.

کلیدواژه‌ها


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