Effect of Mesorhizobium and Thiobacillus bacteria and sulfur on yield and yield components of chickpea (Cicer arietinum L.) and some soil parameters

Document Type : Original Articles

Authors

Sakineh Dastorani

Shahin Shahsavani

Shahrokh Gharanjik

Agriculture College, Shahrood University of Technology, Shahrood, Iran

10.22067/ijpr.v9i2.56126

Abstract

Introduction
Chickpea (Cicer arietinum L.) is a low cost crop in agronomic system of semi arid to arid region overall the world. This crop due to adaptable capacity is caltivated in wide range of environmental conditions and different type of soils. Chickpea seeds contain 24-34% protein, this is 2 to 3 times more than cereals and 14 to 24% more than tuber plants. Chickpea seeds inoculation with rhizhibium cause more N₂ fixation and increase chickpea yield. Mesorhizobium ciceri is one of the rhizobium genus that can have good symbiosis with this crop and fix atmospheric nitrogen up to 60-90% of crop nitrogen requirement after establishment on root crop. Therefore it could be said that biological fertilizers can increase crop production in a sustainable system. Mesorhizobiom can increase chickpea seeds and ultimately increase soil Phosphorus and Zinc concentration. Researchs showed that sulphur deficiency cause decrease seed yield. Nevertheless soil sulphur supply can help seed filling and increase economic yield. The aim of this study was evaluating effect of Mesorhizobium and Thiobacillus bacteria’s and sulfur on yield and yield components of chickpea (Cicer arietinum L.) and some soil parameters.
 
Materials & Methods
In order to study the effect of Mesorhizobium and Thiobacillus bacteria and sulfur on yield and yield components of chickpea (Cicer arietinum L.) and some soil parameter, a field experiment was conducted at Jotai region of Khorasan Razavi province in 2013. This research was conducted as factorial based on randomized complete block design with three replications and 18 treatments. Experimental factors were three levels of sulphur (zero, 500 and 1000 kg ha^-1), thiobacillus bacteria in two level (control and inoculation) and Mesoreizhobium in three levels (control, Mesorizhobium ciceri strain 190 and Mesorizhobium ciceri strain 9). Plot size was 2×4 m² and row spacing and intra-row spacing were 60 and 20 cm, respectively. Irrigation were done at every 14 days. Weed control were done manually. Disease and pest were not recorded during experiment. After harvesting the crop, plant and soil samples from each plots were collected and transported to laboratory evaluation. Analysis of variance were done by SPSS software. Figures were prepared by Excel 2007. Mean comparison of data were done for least significant difference (LSD) at 1 and 5% level of significant.
 
Results & Discussion
Analysis of variance of plant height showed that main effect of sulphur on plant hight were significant at p<0.01 and interaction effect of sulphur and mesorhizibium bacteria on plant hight were significant at p<0.05. These result is consistent with results of Solemani & Asgharzadeh (2010). Analysis of variance of data showed that different species of mesorizhobium bacteria had significant effect at p<0.01 on cheickpea yield. Mean comperision showed that the highest seed yield (1146.73 kg ha^-1) belong to mesorizhobium ciceri 190 and lowest seed yield (971.07 kg ha^-1) belong to mesorizhobium ciceri 9. Scientific reports showed that mesorizhobium inoculation with 25 kg ha^-1 zinc sulphate had significantly increased the number of pods in plant and handred seed weight. Analysis of variance of root length showed that main and interaction effect of treatments were not significant on root length excep in mesorizhobium that were significant at p<0.05. Analysis of variance of seed protein showed that main effect of sulphur, mesorizhobium, sulfhur and mesorizhobium and thiobacillus with mesorizhobium were significant at p<0.01. Role of sulphur on seed protein were well understood. Analysis of variance of seed yield showed that main effect of mesorizhobium and interaction effect of sulphur and thibacillus and sulphur and mesorizhobium on seed yields were significant at p<0.01. Shrivastava et al, (2000) showed that mesorizhobium bacteria inoculation had significant effect on soyabean yield. Analysis of variance of soil sulphur showed that interaction effect of sulphur and thiobacillus had significant effect at p<0.01 on soil sulphur, phosphorus and soil pH but had no effect on soil potassium and electrical conductivity at p<0.05. Main effect of sulphur had significant effect on soil phosphorus and soil pH at p<0.01. However the main effect of mesorizhobium bacteria had significant effect on soil phosphorus at p<0.01. Main effect of thiobacillus had only significant effect at p<0.01 on soil pH. The interaction effect of sulphur and mesorizhobium had significant effect at p<0.01 on soil phosphorus, soil potassium and soil pH at p<0.01, but interaction effect of thiobacillus and mesorizhobium bacteria had only significant effect at p<0.01 on soil phosphorus. Interaction effect of all three factors had significant effect at p<0.01 on soil phosphorus and at p<0.05 on soil sulphur, EC and soil pH.
 
Conclusion
Analysis of variance of data in this research showed that factors in this study had positive and significant effect on agronomic parameters of chickpea and soil physic-chemical properties.

Keywords

Chickpea

Mesorhizobium bacteria

Sulfur

Thiobacillus

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References

1. Afshari, A. 2013. Effect of raizobium bacteria and growth promoter on yiels and yield components of chickpea. Plant Production Technology 13: 71-81. (In Persian with English Summary).
2. Aliehyaei, M., and Behbehanizadeh, A.A. 1994. Soil Chemical Analysis Methods. Technical Journal of Soil and Water Research. Tehran. Iran. (In Persian with English Summary).
3. Al-Karaki, G.N. 2006. Nursery inoculation of tomato with arbuscular mycorrhizal fungi and subsequent performance under irrigation with saline water. Science of Horticulture 109: 112.
4. Amani, F., and Reisei, F. 2007. Effect of sulphur application on amount of soil phosphorus, potassium and zinc after cultivation of two soybean varieties. 10^th Soil Science Congress. Iran. Pp: 434-435. (In Persian).
5. Amani, F., Reisi, F., and Mosavei, S. 2007. Growth and yield of two soya varieties with different sulphur levels on greenhouse condition. 10^th Soil Science Congress. Karaj, Iran. 436-437. (In Persian)
6. Ansori, A., and Gholami, A. 2014. Symbiotic effect of mycorriza, thiobacillus and sulphur on yield and growth of maize. Journal of Soil Manegment and Sustainable Production 4: 114. (In Persian).
7. Bao, L. 1998. The Changes of Fertilizer Structure and Effectiveness in China. Jaingxi Scientific and Techtology Publisher, China.
8. Chapman, H.D., and Pratt, P.F. 1961. Methods of Analysis for Soil, Plants and Waters. University of California. Division of Agriculture. Sciences, p. 309.
9. Altaf, A.I., Khan, N.A., Anjum, I., Diva, M., Abdin Z., and Iqbal, M. 2005. Effect of timing of sulfur fertilizer application on growth and yield of rapeseed. Journal of Plant Nutrition 28: 1049-1059.
10. Besharati, H., and Saleh Rastein, N. 1999. Study on the effect of Thiobacillus bacteria inoculation along with sulphur on increase of available phosphorus absorbtion. Journal of Soil and Water. 13(1): 23-39. (In Persian with English Summary).
11. Daneshi, N., Asgharzadeh, A., and Afshari, M. 2004. Study on the effect of rhizobium inoculation on increasing micro nutrient elements on chickpea cultivation. 9^th Soil Science Congress, Tehran. Soil Conservation and Watershed Research Center of the Country. (In Persian).
12. Ehteshami, M., Aghalikhani, A., Chaichei, M.R., and Khavazei, K. 2007. Effect of phosphate dissolver micro-organisms on some quantity and quality properties of maize seeds at water stress condition. 2^nd National Congress of Sustainable Agriculture. Gorgan, 1-2. (In Persian).
13. Erman, M., Demir, S., Ocak, E., Tüfenkçi, S., Oğuz, F., and Akköprü, A. 2011. Effects of Rhizobium, arbuscular mycorrhiza and they applications on some properties in chickpea (Cicer arietinum ) under irrigated and rainfed conditions: 1- Yield, yield components, nodulation and AMF colonization. Earth Science 122: 14-24.
14. Falahatgar, S., Babaei, B., Besharati, H., and Cheraghi, A. 2011. Effect of different levels of sulphur and thiobacillus inoculation on yield and dry weight, cholorophyll amount and iron and zinc absorbtion of shoot in two soya varieties. First Congress of Science and New Technologies. Zanjan University. (In Persian).
15. Flavio, H., Gutierrez, B., Prystupa, P., and Gustavo, F. 2007. Seed number and yield determination in sulfur deficient soybean crops. Journal of Plant Nutrion 30: 93-104.
16. Garg, N., and Chandel, S. 2011. Effect of mycorrhizal inoculation on growth, nitrogen fixation and nutrient uptake in Cicer arietinum (L.) under salt stress Turkish Journal of Agriculture 4: 35.
17. Karimi, K., Bolandnazar, S., and Ashori, S. 2013. Effect of biological fertilizers and mycorrihza arbascolar on yield, growth properties and green bean qualities. Journal of Agricultural Science and Sustainable Products 23: 392-400. (In Persian with English Summary).
18. Kaplan, M., and Orman, S. 1998. Effect of elemental sulfur containing waste in a calcareous soil in Tukey. Journal of Plant Nutrition 21: 1655-1667.
19. Kochakzadeh, Y., and Malakoti, M.J. 2001. Study on the role of sulphur, thiobacillus, phosphate dissolver and organic matter in supplying phosphorus needs of plant from soil phosphate. Journal of Soil and Water 12: 243-250. (In Persian with English Summary).
20. Khosrojerdi, M., Shahsavani, Sh., Gholipor, M., and Asghari, H.R. 2013. Effect of rhizobium and mycorrhizal fungi inoculation on some nutrient uptake by chickpea at different levels of iron sulfate fertilizer. Electerical Journal of Crop Production 6: 71-82. (In Persian with English Summary).
21. Mahmmodabadi, M., Rashidi, A.L., and Fekri, M. 2013. Effect of alfalfa residue, chicken manure and potassium fertilizer on some soil properties and onion yield. Journal of Soil and Water Agriculture Science and Industry 27: 452-461. (In Persian with English Summary).
22. Majnon Hosseini, N. 1993. Legumes of Iran. Jahad Daneshgahi. Tehran University Publisher.
23. Malakouti, M.J. 2004. Soil Fertility of Arid and Semi-arid Regions. Tarbiat Modarres University, 482 pp.
24. Malcolm, R.E., and Vaghuan, D.A. 1979. Humic substances and phosphatas activities in plant tissues. Soil Biology Chemistry 11: 253-259.
25. Mendel, R. 1997. Molybdenum cofactor of higher plants: Biosynthesis and molecular biology. Planta 203: 399-405.
26. Naderi Erfani, A., Bakhshandeh, A., Nadeian, H., and Alami, K. 2007. Study on the effect of different amount of sulphur and potassium on yield and yield components of canola on cold condition. 10^th Congress of Soil Science, Karaj, Iran, Paradaise of Agriculture and Natural Resources. Tehran University. 17. (In Persian).
27. Olsen, S.R., Cole, C.V., Watanabe, F.S., and Dean, L.A. 1954. Estimation of available phosphorus in soils by extraction with sodiu United States Department of Agriculture Circular 939: 1-19.
28. Saini, V.K., Bhandari, S.C., and Tarafdar, J.C. 2004. Comparison of crop yield, soil microbial C, N and P, N fixation, nodulation and mycorrhizal infection in inoculated and non-inoculated sorghum and chickpea crops. Field Crops Research 89: 39-47. (In Persian with English Summary).
29. Sogut, T. 2006. Rhizobium inoculation improves yield and nitrogen accumulation in soybean (Glycine max) cultivars better than fertilizer. New Zealand Journal of Crop and Horticultural Science 34: 115-120.
30. Shrivastava, U.K., Rajput, R.L., and Dwivedi, M.L. 2000. Response of soybean-mustard cropping system to sulfur and bio-fertilizers on farmer’s field. Legume Research 23: 277-278.
31. Solemani, R., and Asgharzadeh, A. 2010 Effects of Mesorrhizobium noculation and fertilizer application on yield and yield components of rainfed chickpea. Iranian Journal of Pulses Research 1: 1-8 (In Persian with English Summary).
32. Tabatabai, M.A. 1986. Sulfur in Agriculture. American Society of Agronomy Incopration, Madison, Wis., USA.
33. Tisdale, S.L., Nelson, W.L., Beaton, J.D., and Havlin, J.L. 1993. Soil Fertility and Fertilizer. 5^th Mcmillon Publishing Co., New York. 634.
34. Togay, N., Togay, Y., Cimrin, K.M., and Turan, M. 2008. Effect of Rhizobium inoculation, sulfur and phosphorus application on yield, yield components and nutrient uptake in chickpea (Cicer arietinum). African Journal of Biotechnology 7(6): 776-782.
35. Walkley, A.J., and Black, I.A. 1934. Estimation of soil organic carbon by the chromic acid titration method. Soil Science 37: 29-38.
36. Yadegari, M. 2001. Evaluation of Soybean [Glycine Max (L.) Merr] Seeds Inoculation with Bradyrhizobium japonicum on Yield and Yield Components to Select the Best Combination (Inoculant-Cultivar). Tehran University, Thesis of Master of Science in Agriculture.
37. Zapata, F., and Roy, R.N. 2004. Use of Phosphate Rocks for Sustainable Agricultur. Publication of the FAO Land and Water Development Division. pp.117-122.
38. Zeng, Q.F. 1996. Researches on the effect of zinc applied to calcareous soil in cotton field. China Journal of Cotton 23: 21.