شناسایی نماتدهای پارازیت گیاهی جمع‌آوری‌شده از مزارع حبوبات در استان خراسان شمالی

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه فردوسی مشهد

چکیده

به‌منظور شناسایی نماتدهای انگل گیاهی مزارع حبوبات، مجموعاً 41 نمونه ریشه و خاک اطراف ریشه‌های نخود، لوبیا و عدس از مزارع کشت این محصولات در مناطق مختلف استان خراسان شمالی جمع‌آوری و نماتدهای موجود در آن‌ها با استفاده از روش الک و سانتریفیوژ استخراج گردید. پس از تهیه اسلایدهای میکروسکوپی، نماتدهای استخراج شده با استفاده از منابع معتبر علمی مورد شناسایی قرار گرفت. در این بررسی 11 گونه نماتد از هفت جنس متعلق به زیرراسته‌های Tylenchina و Aphelenchina شامل گونه‌های Aphelenchoides limberi، A. spicomucronatus، Aphelenchus avenae، Ditylenchus adasi، D. tenuidens، Filenchus cylindricaudus، F. thornei، Geocenamus tenuidens، Helicotylenchus vulgaris، Pratylenchus neglectus و P. thornei تشخیص داده شد. در بین گونه‌های شناسایی شده، گونه Ditylenchus adasi برای اولین بار از ایران گزارش می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of plant parasitic nematode collected from pulse fields in Northern Khorasan province

نویسندگان [English]

  • Samira Ahmadi
  • Esmat Mahdikhani Moghaddam
  • Sareh Baghaee Davari
Ferdowsi University of Mashhad
چکیده [English]

In order to identify the plant parasitic nematodes of pulse fields in Northern Khorasan province, 41 soil and root samples were collected from rhizosphere of Pisum sp., Phaseoulus sp., Lens sp. during 2011 and 2012. The nematodes were extracted by combined sieving and centrifugal-flotation method of Jenkins (1969) and transferred to glycerin by using method of De Grisse (1969). The permanent slides were prepared and the nematodes were studied by light microscopy. In this study, 11 species from seven genera belonging to Tylenchina and Aphelenchina were identified as follows: Aphelenchus avenae, Aphelenchoides limberi, A. spicomucronatus, Ditylenchus adasi, D. tenuidens, Filenchus cylindricaudus, F. thornei, Geocenamus tenuidens, Helicotylenchus vulgaris, Pratylenchus neglectus, P. thornei. Among these species, Ditylenchus adasi is new record for nematode fauna of Iran.

کلیدواژه‌ها [English]

  • northern Khorasan
  • plant parasitic nematodes
  • Pulse
  • Tylenchina
  • Aphelenchina
1. Ali, S.S., and Askary, T.H. 2001(a). Taxonomic status of phytonematodes associated with pulse crops. Current Nematology 12 (1,2): 75- 84.
2. Ali, S.S., and Askary, T.H. 2001(b). Taxonomic status of nematodes of pulse crops. Nematode taxonomy: Concepts and recent trends. Proceeding of the workshop at Maulana Azad National Urdu University, pp. 197-216.
3. Andrassy, I. 1954. Revision der Gattung Tylenchus Bastian, 1865 (Tylenchidae, Nematoda). Acta Zool. Hung.1, 5-42.
4. Azimi, S., Mahdikhani–Moghadam, E. 2013. Root lesion nematodes associated with faba bean fields in Iran with two new records of Pratylenchus crassi Das & Sultana (1979) and P. teres Khan & Singh (1974). Advanced Crop Science 3 (6): 398-404.
5. Azimi, S., Mahdikhani-Moghadam, E., Rouhani, H., and Rajabi-Memari, H. 2014. The rare genus Apratylenchoides Sher, 1973 (Nematoda: Pratylenchidae) from faba bean in Iran. Archives of Phytopathology and Plant Protection 5 (1):1-7.
6. Bagheri, A.R., and Parsa, M. 2009. Pulses. Jahad daneshgahi Mashhad.
7. Bastian, H.C. 1865. Monograph on the Anguillulidae, or free nematodes, marine, land and fresh water; with descriptions of 100 new species. Transactions of Linnaean Society of London, 25:1-248.
8. Brzeski, M.W. 1991. Review of the genus Ditylenchus Filipjev, 1936 (Nematoda: Anguinidae). Revue de Nematologie 14: 9-59.
9. De Grisse, A.T. 1969. Redescription ou modification de quelqutes techniques utilissisedans L; etude des Nematode phytoparasutaires. Meded Rijksfaculteit der Landbouwwetenschappen Gent, 34: 351-369.
10. Fadavi Khalajlo, G., Mahdikhani Moghadam, E., and Rouhani, H. 2013. Identification of Tylenchina and Aphelenchina nematodes in tomato fields of North Khorasan province. Journal of Plant Protection 27 (2): 169-176.
11. Fadavi Khalajlo, G., Mahdikhani Moghadam, E., and Rouhani, H. 2014. Identification of Ditylenchus species of tomato fields in North Khorasan province. Iranian Journal of Plant Protection Science 44 (2): 183-190.
12. Gritzenko, V.P. 1971. Ditylenchus tenuidens sp. n. and Aphelenchoides curiolis sp. n. (Nematoda, Tylenchidae, Aphelenchoididae) from Kirghizia. Zoologicheskii Zhurnal, 50: 1402-1405.
13. Karegar, A. and Geraert, E. 1998. Descriptions of Filenchus paravesiculosus sp. n. and three other species of the genus Filenchus Andrassy, 1954 (Nemata: Tylenchidae) from Iran. Nematologica, 44: 225-239.
14. Karegar, A. 2006. Identification of plant parasitic nematodes associated with sugar beet fields and their distribution in Hamadan Province, Iran. Iranian journal of Plant Pathology 42 (1): 159-178.
15. Kheiri, A. 1972. Plant parasitic nematodes (Tylenchida) from Iran. Biol .Jb .Dodonaea 40: 224-239.
16. Koochecki, A. 1985. Agronomy in arid zone. Jahad Daneshgahi Mashhad.
17. Jamali, S., Pourjam, E., Kheiri, A., and Damadzadeh, M. 2003. Identification of Aphelenchoides species collected from cereal fields in Isfahan Province. Iranian journal of Plant Pathology 39 (1-2): 17-32.
18. Machado Menten, J.O., Lordello, L.G.E., Tulmann Neto, A., and Ando, A. 1979. Nematodes associated with beans (Phaseolus vulgaris) in Sao Paulo State: preliminary information, Trabalhos apresentados a IV Reuniao Brasileria de Nematologia, 4: 205-212.
19. Mohammad Deimi, A., Ghalandar, M., and Barooti, S. 2008. Study of plant parasitic nematodes on bean in Khomein. Proceeding of the 18th Iranian Plant Protection Congress, Hamedan, Iran. p. 559.
20. Pakniyat, M. 1991. Identification of plant parasitic nematodes of grape in Fars province. MSc. Thesis, College of Agriculture, Tehran University.
21. Pakniyat, M. 2008. Identification of plant parasitic nematodes (Tylenchida) of rapeseed in Fars province. Proceeding of the 18th Iranian Plant Protection Congress, Hamedan, Iran. p. 583.
22. Prakash, A. 1981. Lens culinaris - a new host for root-knot nematode, Meloidogyne javanica in India, National Academy Science Letters 4 (12): 459.
23. Rashid, F., Geraert, E., and Sharma, H.K. 1986. Seinura, Aphelenchoides and Aphelenchus from Brazil (Nematoda: Aphelenchina). Biol. Jb. Dodonaea 54: 30-45.
24. Raski, D. J., and Geraert, E. 1986. Review of the genus Filenchus Andrassy, 1954, and description of six new species (Nemata: Tylenchidae). Nematologica 32: 265-311.
25. Sawadogo, A., Thio, B., Kiemde, S., Drabo, I., Dabire, C., Ouedraoqo, J., Mullens, T.R., Ehlers, J.D., and Roberts, P.A. 2009. Distribution and prevalence of parasitic nematodes of cowpea (Vigna unguiculata) in Burkina Faso, Journal of Nematology 41 (2): 120- 127.
26. Sher, S.A., and Allen, M.W. 1953. C.I.H. Description of Plant Parasitic Nematodes, set. 7, No. 93. Farnham royal, U.K.: Pratylenchus thornei Common Wealth Agricultural Bureaux.
27. Siddiqi, M.R. 1986. Tylenchida (Parasitic of Plants and Insects). Common Wealth Agriculture Bureaux. U.K. 645 pp.
28. Siddiqi, M.R. 2000. Tylenchida (Parasitic of Plants and Insects).2nd ed. CABI publishing. New York.
29. Sturhan, D. 1980. Nematode problems of field beans (Vicia faba), Mitteilungen aus der Biologischen Bundesanstalt fur Land- und Forstwirtschaft. 197: 49-57.
30. Sykes, G.B. 1980. A new species of Nothotylenchus (Nematoda; Neotylenchoidea) from England. Systematic Parasitology 1: 237-239
31. Thorne, G., and Malek, R.B. 1968. Nematodes of the Northen Great Plains, Part1. Tylenchida (Nemata: Secernentea). Agricultural Experiment Station Bull 31: 1-111.
32. Truskova, G.M. 1973. A new species of nematodes of the genus Aphelenchoides Fisher, 1894 (Nematoda: Aphelenchoididae). Parasitologia 7: 188-189. (In Russian).
33. Townshend, J.L., and Anderson, R.V. 1976. Pratylenchus neglectus. C.I.H. Description of Plant Parasitic Nematodes, set. 6, No. 82. Farnham Royal, U.K.: Common Wealth Agricultural Bureaux.
34. Wu, L.Y. 1969. Dactylotylenchinae, a new subfamily (Tylenchidae: Nematoda). Canadian Journal of Zoology 47: 909-911.
35. Yuen, P.H. 1964. Four new species of Helicotylenchus Steiner (Haplolaiminae: Tylenchida) and a re-description of H. canadensis Waseem, 1961. Nematologica 10: 373-387.
CAPTCHA Image