واکنش‌های فیزیولوژیکی گیاه عدس (Lens culinaris Medik) به تنش شوری و محلول پاشی با سالیسیلیک‌اسید

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه یاسوج

چکیده

در بین تنش‌های محیطی، شوری عامل محدودکننده مهمی در تولید حبوبات در دنیا به‌شمار می‌آید. در این پژوهش، برهم کنش کلرید سدیم و اسید سالیسیلیک بر پارامتر های فیزیولوژیک گیاه عدس به‎صورت آزمایش فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در گلخانه مورد بررسی قرار گرفت. گیاهان شامل سه رقم عدس (کیمیا، کرمانشاه و گچساران)، در معرض غلظت های مختلف کلرید سدیم (0، 60 و 120 میلی مولار) و محلول پاشی توسط اسید سالیسیلیک (0، 2/0 و 5/0 میلی مولار) قرار گرفتند. در گیاهانی که تنها در معرض کلرید-سدیم قرار داشتند، در مقایسه با گیاهان شاهد، با افزایش غلظت کلرید سدیم، پارامتر هایی مانند کلروفیل، کاروتنوئید و محتوای نسبی آب کاهش و مقدار قند های محلول، نشت الکترولیت، مالون دی آلدهید، پرولین و تجمع یون سدیم افزایش یافت. اما گیاهانی که در معرض همزمان کلرید سدیم و اسید سالیسیلیک قرار داشتند، در مقایسه با گیاهانی که تنها در معرض کلرید سدیم بودند، در غلظت های یکسان کلرید سدیم، رنگیزه های فتوسنتزی و محتوای نسبی آب آن ها بیشتر و از میزان قند های محلول، نشت الکترولیت، مالون دی آلدهید، پرولین و یون سدیم کاسته شد. این نتایج نشان می دهد که محلول پاشی اسید سالیسیلیک سبب افزایش بردباری به تنش شوری و کاهش اثر های مضر کلرید سدیم در گیاه عدس می شود. همچنین رقم کیمیا در سطوح بالای کلریدسدیم و محلول‌پاشی با 2/0 میلی‌مولار سالیسیلیک اسید بیشترین محتوای نسبی آب، پرولین، قندهای محلول، کلروفیل و کاروتنوئید را داشت.

کلیدواژه‌ها

عنوان مقاله [English]

Physiological responses of Lentil (Lentil culinaris Medik) to salinity stress and foliar application salicylic acid

نویسنده [English]

  • Hamidreza Balouchi
Yasouj University
چکیده [English]

Among the environmental stresses, salinity is a major limiting factor in world legumes production is considered. In this study, sodium chloride and Salicylic acid interaction on growth parameters were investigated in greenhouse with factorial arrangement in randomized complete block design with three replications. The plant consisted of three cultivars of lentil (Kimiya, Kermanshah and Gachsaran), exposed to different concentrations of sodium chloride (0, 60 and 120 mM NaCl) and solution sprayed by salicylic acid (0, 0.2 and 0.5 mM) were used. In plants only exposed to sodium chloride, with the increase of sodium chloride concentration, chlorophylls, carotenoids and relative water content decreased compared to control while, amount of soluble sugar, electrolyte leakage, MDA, proline and amount of sodium and chloride accumulation increased. In plants exposed to sodium chloride and Salicylic acid, compared with those were only exposed to salinity, in the same concentration of sodium chloride photosynthetic pigments and relative water content were higher, and amount of soluble sugar, electrolyte leakage, MDA, proline and amount of Na accumulation decreased compared to plants only exposed to sodium chloride. The result showed that spray of Salicylic acid (as an antioxidant) caused resistance against salt stress and decreased side effects of sodium chloride in Lens culinaris Medik. Kimia variety in foliar application with 0.2 mM salicylic acid and high NaCl levels had the highest relative water content, proline, soluble sugars, chlorophyll and carotenoid.

کلیدواژه‌ها [English]

  • Pulses
  • Osmotic stress
  • Orto hydroxybanzoic acid
  • Soluble sugar
  • Photosynthesis pigments

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