تأثیر متانول بر خصوصیات جوانه‌زنی گیاه لوبیا (Phaseolus vulgaris L. cv. Sadry) تحت تنش خشکی

نوع مقاله : مقالات پژوهشی

نویسندگان

لرستان

چکیده

به‌منظور بررسی اثر متانول بر میزان درصد و سرعت جوانه‌زنی، شاخص بنیه بذر، طول ریشه‌چه، ساقه‌چه، گیاهچه، وزن خشک ساقه‌چه، ریشه‌چه و آندوسپرم مصرفی گیاه لوبیا رقم صدری در شرایط تنش خشکی آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار به‌اجرا درآمد. عامل اول غلظت‌های متانول با 4 سطح (شامل شاهد یا بدون متانول، 5، 10 و 15 درصد حجمی) و عامل دوم خشکی ایجاد شده با پلی‌اتیلن گلایکول 6000 در 4 سطح (0، 3-، 6- و 9- بار) بود. نتایج نشان داد بین سطوح مختلف متانول اختلاف معنی‌داری از نظر درصد و سرعت جوانه‌زنی، طول ریشه‌چه، ساقه‌چه و گیاهچه، وزن خشک ساقه‌چه و ریشه‌چه، شاخص بنیه بذر و آندوسپرم مصرفی وجود داشت (p 0.01). با افزایش غلظت متانول، کاهش معنی‌داری در کلیه صفات مورد بررسی نسبت به سطح شاهد مشاهده شد. تنش خشکی در سطح 9- بار موجب کاهش معنی‌داری (p 0.01) در میزان درصد و سرعت جوانه‌زنی، طول ریشه‌چه، طول گیاهچه، وزن خشک ریشه‌چه و آندوسپرم مصرفی نسبت به دیگر سطوح شد. نتایج نشان داد که در شرایط بدون تنش خشکی، متانول باعث کاهش معنی‌دار در کلیه صفات مورد بررسی شد. در شرایط تنش خشکی، متانول در کلیه سطوح به‌صورت معنی‌داری درصد جوانه‌زنی، طول ریشه‌چه، طول گیاهچه، وزن خشک ریشه‌چه و ساقه‌چه را کاهش داد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of methanol on germination characteristics of bean (Phaseolus vulgaris L. cv. Sadry) under drought stress condition

نویسندگان [English]

  • Hamzeh Amiri
  • ahmad Ismaili
  • nezam armand
lorestan
چکیده [English]

In order to evaluate the effects of methanol on germination percentage, germination speed index, plumule and radicle length, plumule and radicle dry weigh, vigor index and consumed endosperm of bean in condition of water deficit, a factorial experiment done based on completely randomized design with three replications. The first factor was different levels of methanol included 0 (control), 5, 10 and 15 volumetric percentage, and second factor was negative water deficit in four levels of 0, -3, -6 and -9 bar. Results of this study showed that there was a significant difference among different methanol concentrations regarding germination percentage, germination speed index, plumule and radicle length, plumule and radicle dry weight, vigor index and consumed endosperm (p 0.01). Different levels of methanol caused significant decrease on germination characteristics compared with control. Drought stress at -9 bar level significantly decreased germination percentage, germination speed index, radicle length, radicle dry weigh and consumed endosperm compared with other levels. Results showed that in non-stress condition, methanol levels led to significant decrease on germination characteristics compared with control. In drought stress condition, methanol levels caused significant decrease on germination percentage, plumule dry weigh, radicle length and radicle dry weight compared with control.

کلیدواژه‌ها [English]

  • Consumed endosperm
  • Germination rate
  • Seed vigour
  • Water deficit stress

مراجع

1. Agrawal, R.L. 1991. Seed Technology. Second edition, Oxford and IBH press. New York and London, 445 pp.
2. Albrecht, S.L. 1995. Effects of foliar ethanol application on crop yield. Crop Science 35: 1642-46.
3. Aref, F. 2011. Concentration of zinc and boron in corn leaf as affected by zinc sulphate and boric acid fertilizers in a deficient soil. Life Science Journal 8: 26-31.
4. Auld, D.L., Bettis, B.L., Crock, J.E., and Kephart, K.D. 1988. Planting date and temperature effects on germination, emergence, and seed yield of chickpea (Cicer arietinum L.). Journal of Agriculture 80: 909-914.
5. Bagheri, A., Mahmoudi, A., and Ghezeli, F. 2001. Common Bean: Research for Crop Improvement. Publications Jahad University of Mashhad. (In Persian).
6. Bibi, N., Hameed, A., Ali, H., Iqbal, N., Haq, M.A., Atta, B.M., Shah, T.M., and Alam, S.S. 2009. Water stress induced variations in protein profiles of germinating cotyledons from seedlings of Chickpeas genotypes. Pakistan Journal of Botany 41:731-736.
7. David, C. 2010. The effect of gibberellins (GA3 and GA47) and ethanol on seed germination of Rosa eglanteria and Rosa glauca. Journal of Plant Growth Regulation 41: 1-10.
8. De, F., and Kar, R.K. 1994. Seed germination and seedling growth of mung bean (Vigna radiate) under water stress induced by PEG-6000. Seed Science and Technology 23:301-304.
9. Derek Bewely, J., and Black, M. 1994. Seeds Physiology of Development and Germination. 2nd Ed, Pleum press, London, 577 pp.
10. Donohue, K., Rubio De Casas, R., Burghardt, L., Kovach, K., Willis, C.G. 2010. Germination, postgermination adaptation, and species ecological ranges. Annual Review of Ecology, Evolution, and Systematics 41: 293-319.
11. Downie, A., Miyazaki, S., Bohnert, H., John, P., Coleman, J., Parry M., and Haslam, R. 2004. Expression profiling of the response of Arabidopsis thaliana to methanol stimulation. Journal of Phytochemistry 65: 2305-2316.
12. Emmerich, W.E., and Hardegree S.P. 1991. Seed germination in polyethylen glycol solution. Effect of filter paper exclusion and water vapor loss. Journal of Crop Science 31: 454-458.
13. Fabian, A., Jager, K., and Barnabas, B. 2008. Effects of drought and combined drought and heat stress on germination ability and seminal root growth of wheat (Triticum aestivum L.) seedlings. Journal of Acta Biological 52: 157-159.
14. Gamze, O.K.U., Mehmet Demir, K.A.Y., and Mehmet A.T.A. 2005. Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). Turkish Journal of Agriculture 29: 237-242.
15. Gan, Y.T., Miller, P.R., Stevenson, F.C., and McDonald, C.L. 2002. Seedling emergence, pod development and seed yields of chickpea and dry pea in a semi arid environment. Canadian Journal of Plant Science 82: 531-553.
16. Ganjeali, A., Parsa, M., and Khatib, M. 2008. Quantifying seed germination response of chickpea genotypes (Cicer arietinum L.) influenced temperature and drought stress regimes. Agricultural Research: water, soil and plant agriculture 8: 77-88. (In Persian with English Summary).
17. Gout, E., Aubert, S., Blingy, R., Rebeille, P., and Nonomura, A.R. 2000. Metabolism of methanol in plant cells. Plant Physiology 123: 287-296.
18. Hosseinzadeh, S.R. Salimi, A. Ganjeali, A., and Ahmadpour, R. 2012. Effects of foliar application of methanol on growth and root characteristics of chickpea (Cicer arietinum L.) under drought stress. European Journal of Experimental Biology 2(5): 1697-1702.
19. Hosseinzadeh, S.R., Salimi, A., and Ganjeali, A. 2011. Effects of foliar application of methanol on morphological characteristics of chickpea (Cicer arietinum L.) under drought stress. Environmental stresses in crop science 4: 140-150. (In Persian with English Summary).
20. Hosseinzadeh, S.R., Salimi, A., Ganjeali, A., and Ahmadpour, R. 2013. Effects of foliar application of methanol on photosynthetic characteristics chlorophyll fluorescence and chlorophyll content of chickpea (Cicer arietinum L.) under drought stress. Iranian Journal of Plant Biology 5: 116-129. (In Persian with English Summary).
21. Kafi, M., Nezami, A., Hosaini, H., and Masomi, A. 2005. Physiological effects of drought stress by polyethylene glycol on germination of lentil (Lens culinaris Medik.) genotypes. Iranian Journal of Field Crops Research 3: 69-80. (In Persian with English Summary).
22. Khalid, M.N., Iqbal, H.F., Tahir, A., and Ahmad A.N. 2001. Germination potential of chickpeas (Cicer arietinum L.) under saline condition. Journal of Biology Science 4: 395-396.
23. Liga, M.V., Eraso I., and Sturte, G.W. 2003. Effect of ethanol on the growth and development. Seed Science and Technology 21: 427-435.
24. Makhdum, I.M., Nawaz, A., Shabab, M., Ahmad, F., and Illahi, F. 2002. Physiological response of Cotton to methanol foliar application. Journal of Research Pakistan University 13: 37-43.
25. Mehrafarin, A., Naghdi Badi, H., Noormohammadi, G., Zand, E., Rezazadeh, S., and Qaderi, A. 2011. Effects of environmental factors and methanol on germination and emergence of Persian Fenugreek (Trigonella foenum-graecum L.). African Journal of Agricultural Research 6(19): 4631-4641.
26. Mensah, J.K., Obadoni, B.O., Eruotor, P.G., and Onome, F. 2006. Simulated flooding and drought effects on germination, growth and yield parameters of sesame (Sesamum indicum L.). Africian Journal Biology 5: 1249-1253.
27. Michael B.E., and Kaufman M.R. 1976. The osmotic potential of polyethylenglycol-6000. Plant Physiology 51: 914-916.
28. Nadali, I., Paknejad, F., Moradi, F., and Vazan, S. 2010. Effect of methanol on yield and some quality characteristics of sugar beet (Beta vulgaris L.) cv. rasoul in drought and non-drought stress conditions. Journal of Seed and Plant Improvement 26: 95-108. (In Persian with English Summary).
29. Nonomura, A.M., and Benson, A. 1997. The path of carbon in photosynthesis: improved crop yields with methanol. National Academy Science 89: 9794-9798.
30. Ober, E. 2001. The search for drought tolerance in sugar beet. British Sugar Beet Review. 69: 40-43.
31. Opoku, G., Davies, F., M. Zetrio, E.V., and Camble, E.E. 1996. Relationship between seed vigor and yield of white beans (Phaseolos vulgaris L). Journal of Plant Variety Seed 9: 119-125.
32. Pahlevani, A.H., Rashed, M.H., Ghorbani, R. 2008. Effects of environmental factors on germination and emergence of Swallowwort. Journal of Weed Technology 22: 303-308.
33. Ramberg, H.A., Bradley, J.S.C., Olson, C., Nishio, J.N., Markwell, J., and Osterman, J.C. 2002. The role of methanol in promoting plant growth. Plant Biochemistry and Biotechnology 1: 113-126.
34. Tigabu, M., and Oden, P.C. 2001. Effect of scarification, gibberellic acid and temperature on seed germination of two multipurpose albizia species from Ethiopia. Seed Science and Technology 29: 11-20.
35. Veberic, R., Vodnic, D., and Stampar, F. 2005. Influence of foliar-applied phosphorus and potassium on photosynthesis and transpiration of Golden Delicious apple leaves (Malus domestica Borkh.). Journal of Agriculture Slovenia 85: 143-155.
36. Welch, R.M. 1986. Effects of nutrient deficiencies on seed production and quality. Advanced Plant Nutrition 2: 205-247.
37. Zakaria, M.S., Ashraf, H.F., and Serag, E.Y. 2009. Direct and residual effects of nitrogen fertilization, foliar application of potassium and plant growth retardant on Egyptian cotton growth, seed yield, seed viability and seedling vigor. Acta Ecological Science. 29: 116-123.
38. Zeng, Y.J., Wang, Y.R., and Zhang, J.M. 2010 Is reduced seed germination due to water limitation a special survival strategy used by xerophytes in arid dunes. Journal of Arid Environments 74:508-511.
39. Zlatev, Z.S., and Yordanov, I.T. 2004. Effects of soil drought on photosynthesis and chlorophyll fluorescence in bean plants. Bulgharestan Journal of Plant Physiology 30: 3-18.
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